Hypoxia and DNA damage stabilize the p53 protein, but the subsequent effect that each stress has on transcriptional regulation of known p53 target genes is variable. We have used chromatin immunoprecipitation followed by CpG island (CGI) microarray hybridization to identify promoters bound by p53 under both DNA-damaging and non-DNA-damaging conditions in HCT116 cells. Using gene-specific PCR an...

Objective(s):Intermittent hypoxia (IH), caused by obstructive sleep apnea (OSA), could cause hippocampus or neuron damage through multiple signaling pathways, while the underlying mechanisms are still unclear. Thus, the present study aimed to explore the effect of IH on the biological functions of microglia cells. Materials and Methods:Cell proliferation of BV2 cells after exposure to IH were o...

Background Recent researches reveal that manual puncturing of the trophectoderm of blastocyst before vitrification, increase the quality of embryo. However, in any of these studies, the importance of blastocoelic fluid and its impact on the formation of three cell lines is not mentioned. Therefore, in the present study, the effect of blastocoelic fluid reduction before vitrification on survival...

background and aims: acinetobacter baumannii is mostly a cause of septicaemia, pneumonia and urinary tract infections following hospitalization of patients with more severe illnesses. the aim of this study was to determine the prevalence and detection of the most prevalent virulence genes in a. baumannii isolated from hospital infections of two largest hospitals of tehran, iran. materials and m...

salmonella is one of the most important zoonotic pathogens with many virulence factors playing a major role in its pathogenesis. the aims of this study were to detect spva, int2 and invc virulence genes of different salmonella serotypes isolated from various clinical animal and human sources, and to investigate antibiotic resistance patterns among these serotypes. using a pcr assay, a total of ...

conclusions overall, except for pcvd432 and aggr, we detected high heterogeneity of virulence factors among eaec isolates causing diarrhea in children. one set of genes, in the combination, pic- sepa- agg4a, was associated with strong biofilm. results among the 322 e. coli isolated, 23 were identified as eaec. all eaec carried a 630-bp fragment of the plasmid (paa) encoded pcvd432 and aggr gene...

background: apolipoprotein a2 (apoa2) is the second major apolipoprotein of the high-density lipoprotein cholesterol (hdl-c). the study aim was to identify apoa2 gene variation in individuals within two extreme tails of hdl-c levels and its relationship with hdl-c level. methods: this cross-sectional survey was conducted on participants from tehran glucose and lipid study (tlgs) at research ins...

background:epidermal growth factor receptor (egfr) overexpression is a characteristic of several malignancies and could be considered as an excellent target for designing specific inhibitors such as anti-egfr monoclonal antibodies for cancer therapy. drawbacks exerted by large sizes of full-length antibodies have lead to the development of single chain antibodies, which benefit from having smal...

in the current study, molecular typing of 50 erwinia amylovora strains related to different regions in iran was evaluated using multi-locus sequence analysis and variable number of tandem repeats. in the first assay, phylogenetic tree based on partial sequences of recombinase a, sigma factor s and a heat shock protein groel showed significant identity in studied gene sequences. a single nucleot...