Heat-induced epitope retrieval (HIER) is widely used for immunohistochemistry on formalin fixed paraffin-embedded tissue and includes temperatures well above the melting point of paraffin. We therefore tested whether traditional xylene-based removal of paraffin is required on sections from paraffin-embedded tissue, when HIER is performed by vigorous boiling in 10 mM Tris/0.5 mM EGTA-buffer (pH=...

OBJECTIVE The purpose of this study was to correlate the histological diagnosis made during intraoperative frozen section (FS) examination of hysterectomy samples with complex atypical endometrial hyperplasia (CAEH) diagnosed with definitive paraffin block histology. METHODS FS pathology results of 125 patients with a pre- operative biopsy showing CAEH were compared retrospectively with paraf...

The purpose of the present study was to investigate the possibility of immunoelectron microscopic observation of endocrine cells in paraffin-embedded tissues. The procedure, which involves reprocessing from sliced tissues and immunohistochemical staining by colloidal-gold immunolabeling of paraffin sections from paraffin blocks, was able to reveal the fine characteristics of secretory granules ...

Of 1,510 sputum samples examined from 1,016 patients with bronchopulmonary disorders, Nocardia asteroides was isolated from 67 samples by paraffin baiting, as compared with only 30 isolations by the conventional technique of culturing on Sabouraud dextrose agar. This higher efficacy of the paraffin bait technique was found to be statistically significant (P less than 0.001).

A 64-year-old woman died after 12 years of progressive pulmonary disease which was initially diagnosed as sarcoidosis but later correctly identified as mineral oil pneumonia due to insufflation of paraffin instilled as nasal drops. In view of the potential damaging effect of liquid paraffin on the lungs, the current indications for its use must be strongly questioned.

The cycle life and thermal safety of lithium-iron-phosphate (LiFePO4) batteries are important factors restricting the popularization new energy vehicles. study aims to prevent battery overheating, prolong power improve their by discussing heat production LiFePO4 solve problem temperature rise in natural-convection environment cut consumption liquid cooling system. A numerical simulation experim...

A method is described for examination of the larynx in the histopathology laboratory. Using a slicing machine, transverse slices of the whole larynx are obtained from which representative histological samples may be prepared. This method offers the advantages of a complete gross examination of the normal and pathological structures of the larynx supplemented by histological studies using any of...

The first description of oil aspiration pneumonia in man was by Laughlen in 1925. He described 4 cases diagnosed at necropsy: 3 of the patients were infants and 1 was an adult. In 2 cases the administration of liquid paraffin by mouth was incriminated and in the other 2 oily nasal drops had been used. Pinkerton (1927) described 6 cases diagnosed at necropsy, 5 were infants and 1 was a 6-year-ol...