BACKGROUND Bovine tuberculosis is a highly prevalent infectious disease of cattle worldwide; however, infection in the United States is limited to 0.01% of dairy herds. Thus detection of bovine TB is confounded by high background infection with M. avium subsp. paratuberculosis. The present study addresses variations in the circulating peptidome based on the pathogenesis of two biologically simi...

UNLABELLED Determining the viability of bacteria is a key outcome of in vitro cellular infection assays. Currently, this is done by culture, which is problematic for fastidious slow-growing bacteria such as Mycobacterium avium subsp. paratuberculosis, where it can take up to 4 months to confirm growth. This study aimed to identify an assay that can rapidly quantify the number of viable M. avium...

Copyright © 2012 Mitchell V. Palmer et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Mycobacterium tuberculosis, Mycobacterium bovis, Mycobac-terium avium subsp. avium, Mycobacterium avium subsp. paratuberculosis, Mycobacter...

Although the etiology of Crohn’s disease remains unclear, in addition to genetic and other environmental factors, microorganisms have been discussed as possibly playing an important role. With increasing concern about the transmission of infectious diseases from animals to humans, attention has refocused on Mycobacterium paratuberculosis as a candidate organism in the etiology of Crohn’s diseas...

Paratuberculosis is an infectious-contagious disease caused by the bacterium Mycobacterium avium subspecie paratuberculosis (MAP), which affects digestive tract of ruminants. MAP distributed worldwide; however, there are no publications on seroprevalence this in cattle state Sonora. Therefore, objective study was to detect seropositivity Sonora, as well know its apparent prevalence a population...

ELISAs for paratuberculosis employ a preadsorption step with Mycobacterium phlei to diminish unspecific reactions As M. fortuitum is one of the most frequent environmental mycobacteria, the purpose of this pilot study was to evaluate its use as an alternative for the preadsorption in ELISAs for paratuberculosis. Results suggest that M. fortuitum can be an alternative instead of or associated to...

The objective of the work was to evaluate two ELISA’s for detection anti-Map antibodies. 460 serum and fecal samples bovines (n=267), ovines (n=140), goats (n=53) were evaluated. Serums used detecting antibodies using different ELISAs, one in-house ELISA made with low-molecular weight proteins (<100 kD) from antigen ultra-filtrate Map strain 3065, a commercial paratuberculosis diagnost...

BACKGROUND Many studies have been conducted to define risk factors for the transmission of bovine paratuberculosis, mostly in countries with large herds. Little is known about the epidemiology in infected Swiss herds and risk factors important for transmission in smaller herds. Therefore, the presence of known factors which might favor the spread of paratuberculosis and could be related to the ...

The etiology of Crohn's disease in humans is largely unknown. Clinical signs of Crohn's disease partly resemble the clinical picture of Johne's disease in ruminants caused by Mycobacterium avium subsp. paratuberculosis. Because of the high prevalence of these bacteria in (products of) ruminants and their remarkable thermostability, concern has been raised about the possible role of these bacter...

Paratuberculosis (Johne's disease) was suspected in a herd of approximately 300 sheep after weight loss and scouring had increased in adult animals despite repeated treatment with anthelmintics, antibiotics, multivitamins and minerals. The herd is located near Tarhouna city. Herd history revealed that a total of 60 ewes showed clinical symptoms and deaths during the last two years. The last cas...