نتایج جستجو برای: pcr _rflp

تعداد نتایج: 175063  

Journal: :Angewandte Chemie 2005
Haikuo Li Jiehuan Huang Junhong Lv Hongjie An Xiaodong Zhang Zhizhou Zhang Chunhai Fan Jun Hu

The polymerase chain reaction (PCR) revolutionized molecular genetics and has become one of the most popular techniques in modern biological and medical sciences. Owing to the exponential amplification ability of PCR, one could start from even a single copy of target DNA to produce large amounts of DNA copies for sequencing, molecular diagnosis, or genetic analysis. This remarkable amplificatio...

Journal: :Epigenetics 2009
Tomasz K Wojdacz Tanni Borgbo Lise Lotte Hansen

Many protocols in methylation studies utilize one primer set to generate a PCR product from bisulfite modified template regardless of its methylation status (methylation independent amplification MIP). However, proportional amplification of methylated and unmethylated alleles is hard to achieve due to PCR bias favoring amplification of unmethylated relatively GC poor sequence. Two primer design...

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