The split-marker (SM) protocol has become a popular method for production of knockout mutations in fungi. We used Southern hybridization to compare the performance and efficiency SM with more traditional double-crossover intact marker (IM) creating deletions mating type genes Fusarium graminearum. Both methods successfully produced knockouts at rate between 24 75%: mutants efficiently larger co...

INTRODUCTION The ability to screen blood of early stage operable breast cancer patients for circulating tumour cells is of potential importance for identifying patients at risk of developing distant relapse. We present the results of a study of the efficacy of the immunobead RT-PCR method in identifying patients with circulating tumour cells. RESULTS Immunomagnetic enrichment of circulating t...

BACKGROUND/AIM To develop a novel polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) protocol using a new genomic marker sequence and a novel set of restriction enzymes in order to detect and discriminate 2 Echinococcus species, E. granulosus and E. multilocularis, found in formalin-fixed paraffin-embedded (FFPE) human tissues. MATERIALS AND METHODS DNA was isolated...

BACKGROUND PCR-based detection of minimal residual disease (MRD) in neuroblastoma (NB) patients can be used for initial staging and monitoring therapy response in bone marrow (BM) and peripheral blood (PB). PHOX2B has been identified as a sensitive and specific MRD marker; however, its expression varies between tumors. Therefore, a panel of markers could increase sensitivity. METHODS To ident...

Abstract In recent years, tan spot of wheat caused by the fungus Pyrenophora tritici-repentis has become more prevalent in Oklahoma. Experiments were conducted to investigate race structure, disease symptoms and genetic variability P. isolates collected from winter over three decades. Race determination was for 16 based on expression necrosis and/or chlorosis produced differentials. Variability...

Introduction: Although extensive research has been conducted on lung cancer markers, a singular clinically applicable marker has not been found yet. The objective of this study was to evaluate the sensitivity and the specificity of carcinoembryonic antigen (CEA) mRNA and lung-specific X protein (LUNX) mRNA biomarkers in peripheral blood to detect lung cancer individually and simultaneously. Met...

Serological diagnosis provides a robust and effective approach to monitoring controlling small ruminant brucellosis. Brucella melitensis Rev. 1 is live vaccine strain used in prophylactic vaccination against Because the shares identical serological antigens with corresponding field strains, differentiating infected from vaccinated animals (DIVA) responses hamper surveillance campaigns intervent...

Abstract The boll weevil, Anthonomus grandis Boheman (Coleoptera: Curculionidae), is a major pest of commercial cotton (Gossypium hirsutum) in the southern United States and throughout Central South America. Efforts are underway to develop PCR-based diagnostic tool that can be used rapidly accurately differentiate weevils from other weevil species commonly captured pheromone traps. However, qua...

Early in the SARS-CoV-2 pandemic, polymerase chain reaction (PCR) testing was reserved for symptomatic patients. However, many infections were asymptomatic or mildly symptomatic. Positive serologic antibody (Ab) is theorized to be a marker of prior infection, allowing identification true burden disease and establishment links between outbreaks. The objective assess subjects after PCR compare se...