The cycling parameters for BOX A1R PCR were modified and the primer-specific annealing temperature was optimized. Using Trout Lake Escherichia coli isolates, four different template preparation methods were compared. The cell washing, heat lysis, and DNA isolation methods all gave clear and bright band patterns with good reproducibility. The cell washing template preparation method for direct u...

This report describes the application of PCR fingerprinting for the identification of species and varieties of common dermatophytes and related fungi utilizing as a single primer the simple repetitive oligonucleotide (GACA)(4). The primer was able to amplify all the strains, producing species-specific profiles for Microsporum canis, Microsporum gypseum, Trichophyton rubrum, Trichophyton ajelloi...

The validity of taxa around Microsporum canis was evaluated by a combination of phenetic and molecular methods. Morphological and physiological features were compared with results of sequencing of the internal transcribed spacer (ITS) region of the ribosomal operon, polymerase chain reaction (PCR) fingerprinting and amplified fragment length polymorphism (AFLP) analysis. The seven species inves...

Thirteen isolates of Nocardia asteroides from both soils and aquatic samples (lake and moat sediments, as well as scum from activated sludge), together with a type strain and two known clinical isolates of this species, were characterized by repetitive extragenic palindromic-PCR fingerprinting with the BOX-A1R primer. The resulting DNA fingerprint patterns proved to be strain specific, and clus...

Fusarium is a large fungal genus that widely distributed in the environment, mostly known for its plant pathogenicity. Rarely, it involved human pathology, where type of infection caused highly dependent upon portal entry and immune status host. The study at hand aims to summarize routine methods used diagnosing such infections as well more advanced molecular diagnostic methods, techniques can ...