The polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) experiment has the characteristics of low-cost, rapidity, simplicity, convenience, high sensitivity and specificity; thus, many small medium laboratories use it to perform all kinds single nucleotide polymorphisms (SNPs) genotyping works, as a molecular biotechnology for disease-related analysis. However, lack ava...

Bu çalışmada, kedilerin üst solunum yolu enfeksiyonunda Feline herpesvirus 1 (FHV-1)’in rolünün belirlenmesi amaçlandı. Çalışma kapsamında 45 hasta kedinin klinik muayaneleri yapılarak FHV-1 enfeksiyonu yönünden değerlendirildi. enfeksiyonunun bulgularını (hapşırma, gözyaşı ve burun akıntısı, salivasyon) gösterip aşı herhangi bir tedavi uygulanmamış 20 kedi çalışmaya dahil edildi. Kedilerden al...

Although blood culture is considered the gold standard for detection of neonatal infections bloodstream, its result can take from 48-72 h and has low sensitivity. In this study, we determined usefulness panbacterial polymerase chain reaction (PCR) panviral RT-PCR in serum, as tests early diagnosis sepsis. We studied 195 patients with clinical sepsis, up to age 28 days, hospitalized Neonatal Int...

Acetyl-coenzyme A carboxylase α (ACC-alpha) is considered as the key regulatory enzyme in fatty acid biosynthesis. ACC-alpha gene is located on Caprine chromosome 11 and is polymorphic in many goat breeds. In the current study, we aimed to find possible single nucleotide polymorphisms (SNPs) in the exon 1 region of the ACC-alpha gene in Iranian Mahabadi goat breed. Genomic DNA was extracted fro...

Introduction: The novel coronavirus disease 2019 (COVID-19) has huge impact on public health. RT-PCR of respiratory samples is generally accepted confirmatory test which can miss several cases due to various factors. Case description: A 32-years-old male without any co-morbidity presented with complaints cough and fever was negative for Reverse Transcription Polymerase Chain Reaction (RT-PCR) t...

This work develops a loop-mediated isothermal amplification (LAMP) assay that detects the presence of bacterial pathogens (Pasteurella multocida, Mannheimia haemolytica, and Histophilus somni) for bovine respiratory disease complex (BRD) in crude nasal samples. Diagnosing BRD involves physical examination cattle expressing symptoms linked to disease. Unfortunately, these are not unique alone do...

Polymerase chain reaction-restriction fragment length polymorphism (RFLP-PCR) is a technique used to identify single nucleotide polymorphisms (SNPs) based on the recognition of restriction sites by enzymes. RFLP-PCR an easy-to-perform and inexpensive tool for initial analysis SNPs potentially associated with some monogenic diseases, as well in genotyping, genetic mapping, lineage screening, for...

Diagnostic immunoglobulin (Ig) polymerase chain reaction (PCR) clonality analyses need to be simple, reproducible, and rapid. Sucrose and cresol red (gel loading buffer reagents) were added to a routine IgH PCR reaction mix to obviate the need for adding gel loading buffer separately after PCR amplification. Not only did this decrease the time spent after PCR analysis but also gave similar or e...