BACKGROUND Few studies have addressed the timing of cervical cytologic abnormalities and human papillomavirus (HPV) positivity during the course of an infection. It remains largely unknown how infections detected by HPV and cytology wax and wane relative to each other. The aim of this analysis was to assess the longitudinal relationship of abnormal cytology and HPV positivity in a 7-year prospe...

Abstract Avian leukosis virus subgroup J (ALV-J) causes oncogenic disease in chickens China, resulting great harm to poultry production, and remains widespread China. Herein, we employed a cross-priming amplification (CPA) approach nucleic acid detection device establish visual rapid method for ALV-J. The sensitivity of CPA, polymerase chain reaction (PCR) real-time PCR (RT-PCR) was compared, t...

Artificial recombinants can be generated during PCR when more than two genetically distinct templates coexist in a single PCR reaction. These recombinant amplicons can lead to the false interpretation of genetic diversity and incorrect identification of biological phenotypes that do not exist in vivo. We investigated how recombination between 2 or 35 genetically distinct HIV-1 genomes was affec...

Canine hepatozoonosis is a tick-borne protozoan disease spread by hard ticks of the Ixodidae family. Although this illness has been seen in numerous locations Türkiye, its existence Batman and Van provinces yet to be confirmed. The purpose research was look into canine stray dogs from two distinct areas Türkiye using conventional polymerase chain reaction (PCR). Between 2019 2021, blood samples...

The polymerase chain reaction (PCR) is used widely for the in vitro amplification of DNA. The versatility of PCR can be enhanced by the introduction of sequential steps such as nested primer amplifications (1) or re-amplification protocols (2). PCR can also be done on cDNA templates produced by reverse transcription of RNA (3). These protocols require additional opening and closing of the react...

This article discusses the potential of droplet digital polymerase chain reaction (PCR) for diagnostic detection and monitoring allelic load BRAF V600E mutation in circulating cell-free DNA myeloid progenitor cell population bone marrow patients with Langerhans histiocytosis (LCH). Droplet PCR may serve as a useful tool minimal residual disease improve our understanding pathogenesis cells histi...