Genetic variation within and among wild Turkey, Meleagris gallopavo, subspecies was measured using DNA sequencing and PCR-RFLP analysis of a 655 bp portion ofthe mtDNA D-Ioop region. DNA sequencing from 25 individual Eastern, Merriam, Rio Grande and domestic Turkeys revealed 16 polymorphic sites. Parsimony and neighbor-joining analysis did not show support for the four wild Turkey subspecies st...

The aim of this study was to determine the Nosema species by microscopic and molecular method its association with honeybee colony losses (Colony Collapse Disorder) in apiaries Urmia, Northwest Iran. For purpose, samples were collected from 840 colonies kept 120 five different location Urmia. specimens examined for presence spores. After DNA isolation, 16S rRNA gene evaluated using multiplex PC...

Colonization of nontypeable Haemophilus influenzae (NTHi) in nasopharynx causes respiratory tract disease. In 80% of clinical isolates, HMW proteins are the major adhesions and induce protective antibodies in the hosts. Therefore, it can be used as a vaccine candidate. The aim of this study is designing and cloning of the conserved regions of NTHi hmw1 core binding domain.In this study, the sta...

In the current issue of Clinical Chemistry, Braun et al. [1] describe a mutational analysis method that combines primer oligo base extension (PROBE) and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS). This application further extends the use of MS for genetic analysis and suggests a possible role in the clinic. However, what is the long-term potentia...

Here we report on the modification of a genomic sequencing protocol, published by Frommer el al. (6), which allows the determination of the methylation status of cytosine residues on individual chromosomes. The method is based on PCR amplification from chemically modified genomic DNA, in which unmethylated cytosine residues have been converted into uracil by deamination. Sequencing of individua...

It has only recently been discovered that naturally prevailing microorganisms have a notable role in flotation addition to chemical process parameters and overall water quality. This study’s aim was assess the microbial communities relation chemistry zinc copper mineral plant. Due limitations of cultivation-based methods detect fraction total diversity, DNA-based were utilised. However, it DNA ...

T he polymerase chain reaction (PCR) is a rapid procedure for in vitro enzymatic amplification of a specific segment of DNA. Like molecular cloning, PCR has spawned a multitude of experiments that were previously impossible. The number of applications of PCR seems infinite—and is still growing. They include direct cloning from genomic DNA or cDNA, in vitro mutagenesis and engineering of DNA, ge...

We designed oligonucleotide primer pairs to amplify the promoter region, the translated exon sequences, and the flanking intron sequences of all 18 exons of the LDL receptor gene to compare the ability of the PCR single-strand conformation polymorphism (PCR-SSCP) method with semiautomated solid-phase genomic DNA sequencing to detect sequence variations. In 20 apparently unrelated Danish patient...

Asthma is a disease marked by inflammation of airways with an increasing incidence rate worldwide especially among Asian population. Spleen tyrosine kinase (Syk) is known to be involved in regulation of such inflammation response and thereby rendering its inevitable importance among asthma patients. DNA extraction followed by PCR and sequencing was performed for genomic analysis, mRNA analysis ...

Rapid and correct diagnosis is crucial for the management of multidrug resistance (MDR) in Mycobacterium tuberculosis (MTB). The present study aims at rapid diagnosis for identification of multidrug resistance tuberculosis (MDR-TB) using real-time PCR. FRET hybridization probes targeting most prominent four selected codons for rpoB526 and 531 and for katG314 and 315 genes were designed and eval...