نتایج جستجو برای: pcr were performed
تعداد نتایج: 3783070 فیلتر نتایج به سال:
BACKGROUND Cases of infective endocarditis (IE) require prompt etiological diagnosis for effective treatment. Molecular methods can aid in rapid and reliable diagnosis of culture-negative IE cases. We evaluated the utility of 16S rDNA PCR and sequencing in determining the causative agents of IE in valve tissues, especially when specimens were obtained after initiation of antimicrobial therapy. ...
Cholera rapid diagnostic tests (RDT) could play a central role in outbreak detection and surveillance in low-resource settings, but their modest performance has hindered their broad adoption. The addition of an enrichment step may improve test specificity. We describe the results of a prospective diagnostic evaluation of the Crystal VC RDT (Span Diagnostics, India) with enrichment step and of c...
Achieving a rapid microbiological diagnosis is crucial for decreasing morbidity and mortality of patients with a bloodstream infection, as it leads to the administration of an appropriate empiric antimicrobial therapy. Molecular methods may offer a rapid alternative to conventional microbiological diagnosis involving blood culture. In this study, the performance of a new technology that uses br...
We performed a technical and cost-effectiveness analysis of quantitative reverse transcription-polymerase chain reaction (Q-RT-PCR) for the assessment of HER2 in breast cancer. We evaluated 44 frozen and 55 formalin-fixed paraffin-embedded (FFPE) breast carcinoma specimens by Q-RT-PCR, immunohistochemical analysis, and fluorescent in situ hybridization (FISH). Immunohistochemical and FISH analy...
Skin biopsies of 36 patients with erythema migrans and acrodermatitis chronica atrophicans (ACA) before therapy and those of 8 patients after therapy were examined for Borrelia burgdorferi DNA by PCR. Skin biopsies of 27 patients with dermatological diseases other than Lyme borreliosis and those of 10 healthy persons were examined as controls. Two different primer sets targeting 23S rRNA (PCR I...
Background and Objective: Identification of atypical mycobacterium (Non tuberculosis Mycobacterium NTM) is important because of the worldwide propagation of these organisms. Recently, molecular studies have identified the specific loci for mycobacterium species by DNA - finger printing methods, but these methods are time-consuming and expensive. In this study, in addition to hsp65 PCR-RFLP meth...
results all 90 campylobacter isolates confirmed by m-pcr were successfully typed using flaa-pcr-rflp. eleven different types were defined according to flaa-typing method and the rflp patterns were located at three separate clusters in rflp image analysis dendrogram. conclusions campylobacter jejuni isolates significantly showed more variety than c. coli isolates. a relatively low genetic divers...
Background and Aim: Staphylococcus aureus produces many virulence factors, including toxins, immune-modulatory factors, and exoenzymes. The study was performed to determine virulence and resistance-related factors etA, etB, tst, mecA and femA in the S. aureus isolated from clinical samples using Multiplex PCR. Materials and Methods: In this cross - sectional study, a total 60 S. aureus were co...
background and objectives: pcr is a sensitive assay and could be used as an accurate diagnostic method for detecting various types of microorganisms' genome in low concentration in biological specimens. the demand for sensitive, rapid, safe and easy detection of pcr products has led researchers to a combination of this method with elisa. materials and methods: conserved sequences were selected ...
OBJECTIVE This study was performed to investigate the proportion as well as the predictive factors of pathologic complete response in HER2-positive and axillary lymph node positive breast cancer after neoadjuvant paclitaxel, carboplatin plus with trastuzumab (PCH). RESULTS The pCR rate in the breast, axilla and both was 44.3% (39/88), 47.7% (42/88) and 34.1% (30/88), respectively. Patients wi...
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