An emission spectral analysis was carried out on chemiluminescence emitted from UVB-irradiated squalene. The main emission species produced by the transition of (1 delta g) (1 delta g) and (1 delta g) (1 sigma g +) to (3 sigma g -) (3 sigma g -) were found by spectroscopic analysis of the chemiluminescence. When beta-carotene was added to the irradiated squalene, its spectral pattern changed dr...

Luminol (5-amino-2,3,-dihydrophthalazine-1,4-dione) is synthesized and its chemiluminescent properties are explored. Both spectral and physical data indicate that the luminol is sufficiently pure, despite a low yield. Chemiluminescence of luminol is observed in the presence of H2O2 and an oxidant enhancer. The quantum yield of the luminol chemiluminescence is determined to be moderate (0.10 ± 0...

Chemiluminescent reactions of calcium atoms in the metastablePJ and D2 states with CH3I and CD3I were studied in a beam-gas arrangement. Calcium monoiodide spectra associated with transitions from the electronic A Π, B Σ and C Π states to the X Σ ground state were recorded. Total collision and chemiluminescence cross sections were measured. It was found that isotopic substitution in the methyl ...

A new signal amplification system with an auto-inductive mode of action and distinct chemiluminescence output was developed. The system is composed of a unique structural motif that combines a chemiexcitation mechanism with a quinone-methide elimination into a signal building block. As demonstrated with a probe designed to detect fluoride, an auto-inductive chemiluminescence signal amplificatio...

Accurate assessment of reactive oxygen species (ROS) concentrations may help in the diagnosis of infertility. The chemiluminescence technique, which uses a luminometer to measure ROS, is a common method of assessment. A better understanding of the chemiluminescence technique will allow its proper application in reproductive medicine. A wide range of luminometers are available in the market, and...

Polymorphonuclear leucocyte (PMN) functions (migration in vitro, chemiluminescence, O-2 production, binding of chemotactic peptide, and aggregation) were studied in HLA-B27 positive patients with previous yersinia arthritis (YA). PMNs of patients whose disease had been severe showed chemokinetic and chemiluminescence responses significantly higher than the PMNs of those with a mild disease. The...

A chemiluminescence dot blot hybridization assay was used for the detection of B19 parvovirus DNA in human sera by using digoxigenin-labeled probes. The probes were revealed immunoenzymatically by use of anti-digoxigenin Fab fragments conjugated with alkaline phosphatase. The chemiluminescence signal was obtained by reacting the labeled probe-target complex with an enzyme-triggerable dioxetane ...