In eukaryotic cells, the lipid species are frequently distributed asymmetrically between the plasma membrane leaflets. Phosphatidylserine (PS), in particular, often exhibits a distinct transverse asymmetry, being restricted almost exclusively to the inner leaflet. In the past years, several proteins were suggested to transport lipids between the leaflets of a membrane, and to potentially influe...

P-glycoprotein (Pgp; mouse MDR3) was expressed in Pichia pastoris, grown in fermentor culture, and purified. The final pure product is of high specific ATPase activity and is soluble at low detergent concentration. 120 g of cells yielded 6 mg of pure Pgp; >4 kg of cells were obtained from a single fermentor run. Properties of the pure protein were similar to those of previous preparations, exce...

The effect of P-glycoprotein (Pgp) and/or CYP3A on the disposition of xenobiotics has been extensively investigated and is often of interest during drug discovery lead optimization. We have previously described a monkey pharmacokinetic screen to rapidly estimate absorption and first-pass extraction. In the present work, this monkey screen has been expanded to include an assessment of Pgp/CYP3A ...

The compartmentalization and transport of proteins and solutes across the endothelium is a critical biologic function altered during inflammation and disease, leading to pathology in multiple disorders. The impact of tissue damage and subsequent extracellular matrix (ECM) fragmentation in regulating this process is unknown. We demonstrate that the collagen-derived matrikine acetylated proline-g...

In this project, we investigated diierent possible approaches for Java-capable web browsers to provide exible access control to applets, and then we designed, implemented, and tested our approach. Our approach uses PGP to do authentication and to provide privacy. We modify the class loader to use PGP to authenticate (and decrypt) a signed (and encrypted) applet. The security manager is also mod...

Domestic production of tamarind has decreased in the country due to diseases. Tests were made on individual efficacy botanical extracts like plant growth promoter (PGP) and oriental herb nutrient (OHN), as well Trichoderma harzianum Rifai a possible biocontrol agent against major fungal Using Randomized Complete Block Design (RCBD), field setup was established with five treatments: untreated (n...

Pregnant women frequently complain of low back discomfort associated with their pregnancies. On quality life, it could have a detrimental effect. Pregnancy-related pain (LBP) and pelvic girdle (PGP) are substantial direct indirect expenditures. Evidence addressing strategies to treat prevent these illnesses needs be clarified. This review aimed examine the connection between exercise, LBP, PGP....

BACKGROUND The incidence of pelvic girdle pain (PGP) in pregnancy is wide ranged depending on definition, the utilised diagnostic means, and the design of the studies. PGP during pregnancy has negative effects on activities of daily living and causes long sick leave, which makes it a major public health issue. Our objectives were to explore the frequency of sick leave in pregnancy due to PGP, a...

Tc-99m sestamibi is a substrate of P-glycoprotein (Pgp) that has been proposed for use as a functional imaging agent for the multidrug resistance-1 phenotype. In vitro, retention of sestamibi by cells that overexpress Pgp can be modified by the presence of Pgp antagonists. In a Phase I trial of the Pgp reversal agent PSC 833, we show that the effects of this reversal agent can also be demonstra...