A 45-kDa ribonuclease (RNase) was purified from dried fruiting bodies of the wild mushroom Amanita hemibapha. It was adsorbed on DEAE-cellulose, S-sepharose, and finally purified on Superdex 75. The RNase exhibited maximal RNase activity at pH 5 and in a temperature range between 60-70°C. It demonstrated no ribonucleolytic activity toward four polyhomoribonucleotides. The amino acid sequence an...

Phosphatidylethanolamine (once given the trivial name ‘cephalin’) is usually the second most abundant phospholipid in animal and plant lipids and it is frequently the main lipid component of microbial membranes. It can amount to 20% of liver phospholipids and as much as 45% of those of brain; higher proportions are found in mitochondria than in other organelles. As such, it is obviously a key b...

A simple, RP-HPLC method was established for determining moxifloxacin and ketorolac in pharmaceutical formulations. Moxifloxacin, ketorolac and their degradation products were separated using C8 column with methanol and phosphate buffer pH 3.0 (55:45 v/v) as the mobile phase. Detection was performed at 243 nm using a diode array detector. The method was validated using ICH guidelines and was li...

This study was performed to investigate the role of pH and temperature postmortem, and to demonstrate the importance of these factors in determining meat quality. Postmortem pH 45min (pH at 45 min postmortem or initial pH) via analysis of Pearson's correlation showed high positive correlation with pH change pH c24 (pH change from pH 45min to pH 24h postmortem). However, postmortem pH after 24 h...

The present study was undertaken to elicit the temporal sequence of changes in cardiovascular and respiratory function during aestivation. Twelve lungfish (2-6 kg) equipped with ECG electrodes, arterial and buccal cannulae, were studied while aestivating in mud or in artificial cloth-bag nests. The periods of observation ranged from 0̂ 5 to 9-5 months. The mean arterial blood pressure gradually ...

Effects of pH, temperature, and syrup concentration on color formation in glucose syrups were studied and the shelf life of syrups under various conditions was estimated. Temperatures of 5, 25, and 45 ºC, pH values of 4, 5, and 6 as well as concentrations of 30, 40, 50, 60, 70, and 80 ºBrix were examined. After 26 weeks no significant color changes were observed at 5 ºC. At 45 ºC, color formati...

A cellulase with wide range of pH resistance and high salt tolerance was isolated from the digestive gland of the oyster Crassostrea rivularis living in mangrove forests. The 27 kDa cellulase named as CrCel was purified 40.6 folds by anion exchange chromatography and extraction from the gel after non-reducing sodium dodecylsufate-polyacrylamide gel electrophoresis. The specific activity of the ...

An expression vector for bovine NADH-cytochrome b5 reductase was used for site-directed mutagenesis of lysine 110, the residue previously implicated in NADH interactions with this flavoprotein. Replacement of this basic residue with an uncharged glutamine resulted in an increase of 3 orders of magnitude in the Km for NADH and a decrease in kcat of an order of magnitude, strongly implicating lys...

The development of an optical pH sensor based on immobilization of quinizarin (1,4-dihydroxyanthraquinone) on a triacetylcellulose membrane was described. The resulting membrane exhibited fast color change from yellow to violet, while changing the pH in alkaline region. The immobilized quinizarin showed a broader dynamic range from (pH 9.4-11.4) with respect to free form (pH 8.8-10.4). The sens...