The polo-box domain of the budding yeast polo kinase Cdc5p plays an essential role for targeting the catalytic activity of Cdc5p to spindle pole bodies (SPBs) and cytokinetic neck-filaments. Here, we report the isolation of Bbp1p as a polo-box interacting protein by a yeast two-hybrid screen. Bbp1p localizes to the periphery of the central plaque of the SPB and plays an important role in SPB du...

The southern pine beetle (SPB; Dendrodonus frontalis Zimmermann) has been a major source of timber and income loss to nonindustrial private forest landowners in the southern region of United States. Efforts to promote forest health through prevention and control must identify new ways to reach the sociologically diverse and spatially dispersed nonindustrial privote landowners in this region. Th...

BACKGROUND Single-use rocking-motion-type bag bioreactors provide advantages compared to standard stirred tank bioreactors by decreased contamination risks, reduction of cleaning and sterilization time, lower investment costs, and simple and cheaper validation. Currently, they are widely used for cell cultures although their use for small and medium scale production of recombinant proteins with...

The yeast spindle pole body (SPB) is the functional equivalent of the mammalian centrosome. The half bridge is a SPB substructure on the nuclear envelope (NE), playing a key role in SPB duplication. Its cytoplasmic components are the membrane-anchored Kar1, the yeast centrin Cdc31, and the Cdc31-binding protein Sfi1. In G1, the half bridge expands into the bridge through Sfi1 C-terminal (Sfi1-C...

l.~rio, P.L., Jr., 1986. Growth--different iat ion balance: a basis for understanding southern pine beetle--~ree interactions. For. Ecol. Manage., 14:259-273 . Interrelationships between the southern pine beetle (SPB), Dendroctonus frontalis Zimm.) and its host pines are explained in terms of the growth--differentiat ion balance concept. A general hypothesis is proposed based on growth--differe...

Spindle pole bodies (SPBs), like nuclear pore complexes, are embedded in the nuclear envelope (NE) at sites of fusion of the inner and outer nuclear membranes. A network of interacting proteins is required to insert a cytoplasmic SPB precursor into the NE. A central player of this network is Nbp1 that interacts with the conserved integral membrane protein Ndc1. Here, we establish that Nbp1 is a...

The influence of particles properties on biofilm structure, reactor performance, and energy consumption for denitrifying fluidized bed bioreactors (DFBBRs) using maxi-blast plastic (MX), multi-blast plastic (MB), natural zeolite (NZ), and lava rock (LR) was investigated. The work showed that the particles with sphericity of 0.9 (MB and NZ) maintained a fluffy protruding biofilm and achieved sli...

Optimizing bioprocesses requires an in-depth understanding, from a bioengineering perspective, of the cultivation systems used. A characterization is typically performed via experimental or numerical methods, which are particularly well-established for stirred bioreactors. For unstirred, non-rigid such as wave-mixed bioreactors, methods prove to be problematic, often only simplified geometries ...

The number of cancer cases and deaths are increasing year by [1]. high cost treatment the guilt patients relying on caregivers for long-term care leads to a self-perceived burden (SPB) among affecting their quality life, even cause suicidal ideation [2]. objective this study is determine level its associated factors in Fourth Hospital Hebei Medical University, China. A sample size 977 was recru...

We have used time-lapse digital imaging microscopy to examine cytoplasmic astral microtubules (Mts) and spindle dynamics during the mating pathway in budding yeast Saccharomyces cerevisiae. Mating begins when two cells of opposite mating type come into proximity. The cells arrest in the G1 phase of the cell cycle and grow a projection towards one another forming a shmoo projection. Imaging of m...