separation of some biogenic amines via rp-hplc using mixed micellar mobile phase was investigated. the compounds were derivatized before hand by dansyl chloride as a chromophoric reagent. appropriate conditions for separation, were determined by studying factors such as temperature, type and percentage of organic modifier, concentration of surfactants (sds and brij-35) and the ph of the mobile ...

HPLC/ESI-MS analyses of alcohol solutions of ampicillin and amoxicillin have shown that the two drugs react very readily with alcohols (methanol and ethanol) at room temperatures, without the presence of any catalyst. Products of methanolysis were detected even when methanol was used as a mobile phase, in HPLC/ESI-MS analysis of a water solution of ampicillin and amoxicillin. Therefore, neither...

A improved, simple and economic reverse phase high performance liquid chromatography (RP-HPLC) method has been developed and validated as per ICH guidelines for the quantification of acetic acid present in Gefitinib as an solvent impurity. Validation studies demonstrated that the proposed RP-HPLC method is simple, specific, rapid, reliable and reproducible. The high recovery and low relative st...

Two unknown impurities were detected in verapamil hydrochloride bulk drug using isocratic reversed-phase high performance liquid chromatography (HPLC). These impurities were isolated by preparative HPLC. Spectral data for the isolated impurities were collected. Based on the spectral data derived from two-dimensional nuclear magnetic resonance (2D-NMR) spectroscopy and mass spectrometry (MS), im...

In this study, hollow fiber liquid phase microextraction (HF-LPME) coupled with highperformanceliquid chromatography (HPLC) with UV detection was applied for preconcentrationand determination of Diclofenac sodium in biological fluids. Parameters affecting the extractionprocess including pH of donor phase and acceptor phase, type of extraction solvent, stirring rate,extraction time, and salt add...

A multicomponent pharmaceutical that contains loratadine, paracetamol, and pseudoephedrine was quantified using HPLC-PDA. The three analytes were well-separated in the dosage form on a C-18 column gradient mobile phase. quality by design strategy followed to achieve challenging separation. Screening optimization steps carried out investigate effect of many factors studied responses with minimum...

a sensitive high performance liquid chromathography (hplc) analytical procedure was developed for the quantitive determination of trimethoprim (tm* and sulphamethoxazole (sm) in commercial dosage forms. c18 analytical column (stainless steel, 25 cm * 4.6 mm i.d.) was packed with 5-*m particles of the reversed phase material and used for assays. mobile phase containing 0.025 m sodium phosphate a...

Naringenin and eriodictyol are chiral flavanones widely present in citrus fruits and herbal products. Pharmacological interest in the two flavanones is well known. Due to the chiral carbon atom, the compounds always exist in the racemic form. The present study reported a stereospecific HPLC method for the enantioseparation of naringenin and eriodictyol, which was performed on an amylase-based c...

A sensitive (0.01-1 nmol) method has been developed for the analysis of polyamines in higher plant extracts based on high performance liquid chromatography (HPLC) of their benzoyl derivatives (Redmond, Tseng 1979 J Chromatogr 170: 479-481). Putrescine, cadaverine, agmatine, spermidine, spermine, and the less common polyamines nor-spermidine and homospermidine can be completely resolved by rever...

The present study describes development and subsequent validation of high performance liquid chromatographic method (HPLC) in comparison with spectrophotometric methods (classic, first, second and third order derivative) for determination of pure cilazapril in substance and pharmaceutical preparation. The main aim of this study was to find the method suitable not only for determination of cilaz...