DL-1-(2,3-Dihydroxypropyl)thymine was prepared by Hilbert-Johnson reaction of 2,4-dinethoxy-5-methylpyrimidine with allyl bromide followed by the osmium tetroxide catalyzed hydroxylation of the l-allyl-4-methoxy-5-methylpyrimidin-2-one obtained as an intermediate. The D-glycero enantiomer, R-1-(2,3-dihydroxypropyl)thymine and the corresponding 1-substituted uracil derivative were prepared from ...

Calcium phosphates are the main inorganic components of bone and tooth tissues. The nucleation of calcium phosphates is thought to proceed by aggregation of so called prenucleation clusters (PNCs), which are already present in body fluids before nucleation. This nucleation pathway cannot be fitted in the frame of the Classical Nucleation Theory. We investigated the structure and verified the st...

A general one-step strategy is developed for the synthesis of hexofuranosyl 1-phosphates starting from new unprotected glycofuranosyl donors. It required first the preparation of new 1-thiohexofuranosides bearing a thioimidoyl heterocycle as a leaving group. The presence of sulfur and/or nitrogen atom(s) on the aglycon allowed remote activation of these thioglycofuranosides by anhydrous phospho...

The pool of chemical energy in an organism represented by high-energy compounds can be assessed by means of adenosine triphosphate (ATP) determination in whole blood and tissues. The elegant manner for the determination of adenosine phosphates (ATP, ADP, AMP) in a single assay is offered by the technique of capillary zone electrophoresis. For this purpose, the BioFocus 3000 Capillary Electropho...

The use of inductively coupled plasma optimal emission spectroscopy as a detector for the high-performance liquid chromatographic analysis of inositol phosphates is studied. It is found that separation of different inositol phosphates with a mobile phase consisting of tetraethylammonium (0.14%, w/v), methanol (5%, v/v), and formic acid (0.18%, w/v) may be obtained on a PRP-1 column with an anal...

We synthesized Al-containing acidic and basic (alkaline) sodium aluminum phosphates (SALPs) which are FDAapproved leavening and emulsifying agents, respectively, and used them to determine the oral bioavailability of aluminum incorporated in selected foods. We selected applicable methods from published syntheses (patents) and scaled them down ( 3000and 850-fold) to prepare 300–400 mg of each SA...