Selection of poultry for fast growth rate is often accompanied by a reduction in specific immune responses or increased disease susceptibility. In this study, 17-wk-old male turkeys from each of four closed genetic lines, a randombred control (RBC) line and its subline (F) selected for increased 16-wk BW, and another RBC line and its subline (E) selected for increased egg production, were teste...

T-cell activation in response to interleukin-12 (IL-12) is mediated through signaling events that include the tyrosine phosphorylation of STAT4. IL-12 responsiveness and the ability of IL-12 to activate STAT4 is different in T cells induced to differentiate into a Th1 or Th2 phenotype. In this report, we show that STAT5, STAT1alpha, and STAT1beta, in addition to STAT4, are tyrosine phosphorylat...

Factors influencing basophil production from the bone marrow of ovalbumin (OA)-sensitized guinea pigs have been examined in vitro. Autologous co-cultures of marrow and spleen cells from OA-immune animals contained significantly higher numbers of basophils after 7 d of liquid culture in the presence of OA, compared with control co-cultures or with marrow cultures alone (P < 0.005). Basophils inc...

The murine T lymphoma line, LBRM-33 1A5, requires synergistic signals delivered by phytohemagglutinin (PHA) and interleukin 1 (IL1) for activation to high level interleukin 2 production. The activation signals provided by PHA and IL1 were replaced by the Ca2+ ionophore, ionomycin, and the phorbol ester, 120-tetradecanoylphorbol 13-acetate (TPA), respectively. These observations upported a ...

We have previously shown that concanavalin A (ConA) induction of suppressor cell activity is impaired in patients with lepromatous leprosy (LL). In this study, we demonstrated that the proportion of cells bearing the Leu8 antigen (associated with suppressor-inducer cells) is low in LL patients and tends to normalize during the erythema nodosum leprosum (ENL) episode. Antigen-induced suppressor ...

By YALE RABINOWITL AND ALBERT A. D rz With the technical assistance of Tina Lubrano, Inge Schimo and Betty A. Wilhite II ESULTS OF A STUDY OF CHANGES in lactic dehydrogenase (LDH) occurring in cultures of normal human lymphocytes were reported recently.’ It was shown that lymphocytes in cultures without phytohemagglutinin (PHA) showed little change in total LDH activity or in isozyme patterns; ...

The concept whether immune reactivity was intrinsically related to risk of recurrence was examined in 307 patients with rectal colon cancer, including 181 patients with primary resectable cancer, 48 patients with advanced primary operable cancer, and 78 patients with recurrence. Immune reactivity was measured by 2,4-dinitrochlorobenzene (DNCB) skin tests (219 patients) and by in vitro immunopar...

OBJECTIVES In mammals, the postnatal development of the gastrointestinal tract is characterized by vast structural and functional changes. Using a suckling rat model, we investigated whether red kidney bean lectin, phytohemagglutinin (PHA), a potent gut mitogen in adult rats, can accelerate the growth and maturation of the gastrointestinal tract. METHODS At either 10 or 14 days of age, suckli...

Syringomycin E (SR-E) is a low molecular weight bacterial lipodepsipeptide with antifungal properties. Owing to immunosuppressive activities of such compounds as cyclosporine, FK506 and rapamycin, we studied the effect of SR-E on proliferation of human blood lymphocytes in vitro. SR-E, by itself, had no effect but the mitogen-induced lymphocyte proliferation was significantly suppressed. The su...

In vitro radiation survival of peripheral blood T lymphocytes was studied in 1 5 clinically normal adults and 4 patients with Fanconi’s anemia. Tritiated thymidine incorporation in a whole blood lymphocyte stimulation test (1ST) and a newly developed whole blood T-lymphocyte colony assay were used to measure lymphocyte blastogenesis and colony formation in response to phytohemagglutinin (PHA) o...