The present study compared in vivo protein digestion a miniature pig model with the dynamic vitro system DiDGI®, using three digestive compartments (stomach, duodenum, and jejunum + ileum). Two soya-based meals—commercial soya milk tofu—were studied, each same macronutrient content but different macrostructures. Our aim was to first deduce from experiments pigs key parameters such as gastric pH...

PKC is involved in mediating the tonic component of gastrointestinal smooth muscle contraction in response to stimulation by agonists for G protein-coupled receptors. Here, we present pharmacological and immunohistochemical evidence indicating that a member of the novel PKC isoforms, PKC-delta, is involved in maintaining muscarinic receptor-coupled tonic contractions of the guinea pig ileum. Th...

A series of novel N-substituted 2-methyl imidazole derivatives have been synthesized, and their in vitro antispasmodic activity was assessed on contractions of isolated guinea pig ileum, induced by acetylcholine (5× 10M to 1× 10M), and compared with the effect of atropine. All the prototypes were synthesized and confirmed by their FTIR, HNMR, MASS and elemental spectral data. Antispasmodic acti...

preganglionic motoneurones in the medulla of the cat. J Physiol (Lond) 258: 187-204, 1976 11. Curtis DR, Eccles JC: Synaptic action during and after repetitive stimulation. J Physiol (Lond) 150: 374-398, 1959 12. Auerbach AA: Transmitter release at chemical synapses. In Structure and Function of Synapses, edited by GC Pappas, DP Purpura. New York, Raven Press, 1972, pp 137-159 13. Dennis MJ, Ha...

preganglionic motoneurones in the medulla of the cat. J Physiol (Lond) 258: 187-204, 1976 11. Curtis DR, Eccles JC: Synaptic action during and after repetitive stimulation. J Physiol (Lond) 150: 374-398, 1959 12. Auerbach AA: Transmitter release at chemical synapses. In Structure and Function of Synapses, edited by GC Pappas, DP Purpura. New York, Raven Press, 1972, pp 137-159 13. Dennis MJ, Ha...

Twenty-three structural analogs of the leukotriene components of slow reacting substance of anaphylaxis (SRS-A), in which the polar regions of the leukotriene were systematically modified, were tested for their contractile activities on guinea pig pulmonary parenchymal strips and guinea pig ileum. The structural modifications allowed evaluation of the separate contributions of the four polar un...

Poole DP, Furness JB. PKC -isoform translocation and enhancement of tonic contractions of gastrointestinal smooth muscle. Am J Physiol Gastrointest Liver Physiol 292: G887–G898, 2007. First published December 7, 2006; doi:10.1152/ajpgi.00222.2006.—PKC is involved in mediating the tonic component of gastrointestinal smooth muscle contraction in response to stimulation by agonists for G protein-c...

in order to investigate the effects of dimethylamino substituent at position 2 of the dihydropyridine nucleus on activity, starting from dialkyl l ,4-dihydro-2,6-dimethyl-4- (l-benzyl-2-alkylthio-5-imidazolyl)-3,5-pyridinedicarboxylates (5a-f) which their synthesis and effects as calcium channel antagonist on guinea-pig ileum has been reported previously, dialkyl l,4-dihydro-2-[2-(dimethylamino...

BACKGROUND AND OBJECTIVES The current investigation was carried out to explore the pharmacological basis of the crude extract of Conyza bonariensis (Cb.Cr) for its use in constipation and diarrhea. MATERIALS AND METHODS The plant extract of Conyza bonariensis (C. bonariensis) was prepared, isolated guinea-pig ileum and rabbit jejunum preparations were used to evaluate its gut modulator effect...

Casein, a protein found in milk of several species, is divided into different chains from 19 to 25 kDa. Casein is also considered as a source of amino acids and generating peptides with biological activities such as opiate, immunostimulating, antibacterial, peptidase inhibitors, among others. In this work, Sephadex G-10 chromatography followed by high-performance liquid chromatography isolation...