This research work aimed to enhance the concentration of some bioactive compounds in culantro (Eryngium foetidum L.) plantlets through gamma irradiation (0.0, 10.0, 20.0 and 40.0 Gy range) treatments. Gamma irradiation at 40.0 Gy significantly stimulated total phenolic, flavonoids, tannin and saponin contents. The effect of irradiation on antioxidant properties of culantro extracts was investig...

We developed sugarcane plants with improved resistance to the sugarcane borer, Diatraea saccharalis (F). An expression vector pGcry1Ac0229, harboring the cry1Ac gene and the selectable marker gene, bar, was constructed. This construct was introduced into the sugarcane cultivar FN15 by particle bombardment. Transformed plantlets were identified after selection with Phosphinothricin (PPT) and Bas...

Frequency of somaclonal variation in embryogenic calli and plants of Olea europaea L cv.Kroneiki derived from somatic embryogenesis were examined by randomly amplified polymorphic DNA (RAPD) analysis. Radicle and cotyledon (proximal part) of mature zygotic embryo were cultured in OMc medium supplemented with IBA (5 mgL-1) and 2-ip (0.5 mgL-1). Somatic embryogenesis was induced in calli of radic...

Abstract The black orchid ( Coelogyne pandurata Lindley) is one of the native Indonesian species. In its natural habitat, existence threatened by continuous forest exploitation. Coveted in global market for hybridization purposes, still considered difficult to be grown cultivation and may take years flower. Its conservation breeding carried out through a combination tissue culture mutagen appli...

DNA methylation is believed to play important roles in regulating gene expression in plant growth and development. Paulownia witches' broom (PaWB) infection has been reported to be related to gene expression changes in paulownia plantlets. To determine whether DNA methylation is associated with gene expression changes in response to phytoplasma, we investigated variations in genomic DNA sequenc...

PENG, Z. S., C. K. LUO, P. CAI, Z. C. MAO, C. Y. KANG and J. YANG, 2007. Rapid propagation of the medicinal plant Pinellia ternata by in vitro leaves culture. Bulg. J. Agric. Sci., 12: 01-06 Pinellia ternata (Thunb) Breit is an important medicinal plants. Its tubers,being called as Banxia in Chinese words, were a crude drug of traditional Chinese medicine and some pharmaceutical products. For s...

A protocol has been devised for enhanced in vitro regeneration of critically endangered Mantisia spathulata Schult. and Mantisia wengeri Fischer. Highest Bud Forming Capacity (BFC) of 6.10 +/- 0.55 with an average of 19.93 +/- 3.19 roots was obtained for M. spathulata within 5-6 weeks in Murashige and Skoogs (MS) medium supplemented with a combination of 10.0 microM of N6-benzyladenine (BA) and...

the purpose of this study was to compare rooting methods in tissue culture regenerated-clones in sugar beet and their adaptation to environmental conditions and determine clones ploidy stability. two kinds of explants including shoot apex of seedlings and inflorescence stem pieces were used for preparation and propagation of the clones in media. in the next step, three rooting and adaptation me...

A large number of micropropagated plantlets of banana, Musa acuminata var. Nanjanagudu Rasabale (NR), that were developed from axillary shoot bud explants over 10 years ago were screened for genetic variation, if any, using RAPD (Random Amplified Polymorphic DNA) and ISSR (Inter-Simple Sequence Repeats) markers. Of the 4000 in vitro plantlets, 11 were used for screening that involved shoot cult...

Aloe plants can be grown autotrophically in a maintenance-free, sterile, ex vitro environment. Excel, 15-5-15 plus minors fertilizer formulation and Plant Preservative Medium (PPM) biocide was used as a very economical basal medium. In vitro micropropagated plantlets were regenerated in Excel media with 2 g /L sucrose and 2.2 μM 6-Benzylamino purine (BA) and used to initiate ex vitro cultures i...