Three monoclonal antibodies to cs2-plasmin inhibitor (a2PI) were characterized. The first, JTPI-1 , was directed against the reactive site of a2Pl and inhibited antiplasmin activity by interfering with the formation of a2Pl-plasmin complexes. The avidity of JTPI-1 to the preformed a2Pl-plasmin complex was markedly lower than that to free a2Pl. which made this antibody useful for measuring the f...

teolytic enzyme plasmin (fibrinolysin) is supposed to be present in normal blood plasma in an inactive form, called plasminogen (proplasmin). Anxiety, strenuous exercise, hemorrhage, burn , t rauma (‘ ‘tourniquet shock’ ‘) and epinephrine admmistration have heels found to produce increased fibrinolytic activity (plasmin formation) in the blood. 3 The physiologic mechanism of activation of propl...

Mice deficient in plasminogen, the precursor of plasmin, show completely arrested healing of tympanic membrane (TM) perforations, indicating that plasmin plays an essential role in TM healing. The activation of plasminogen to plasmin is performed by two plasminogen activators (PAs), urokinase-type PA (uPA) and tissue-type PA (tPA). To elucidate the functional roles of PAs in the healing of TM p...

Three Fragment D species (D1, D2, D3) were isolated with time from a plasmin digest of fibrinogen and had molecular weights of 92,999, 86,000 and 82,000 by summation of subunit molecular weights from sodium dodecyl sulfate polyacrylamide gel electrophoresis. Their molecular weights by sedimentation equilibrium ultracentrifugation were 94,000 t87,000, 88,000 to 82, 000, and 76,000 to 70,000 depe...

A new procedure for the synthesis of cyclohexanone-based inhibitors of serine proteases is reported. In this procedure the reactive ketone functionality is carried through the synthesis in masked form as a TBDMS-protected alcohol. Deprotection followed by oxidation of the alcohol generates the final form of the inhibitor. Two new inhibitors, which interact with the S1-S3 and S2' subsites of pla...

Porcine tissue-type plasminogen activator (t-PA) increases the binding of 125I-glu-plasminogen to clots made from human plasma or purified fibrinogen in a time and t-PA concentration dependent fashion. The accumulation of plasminogen was faster and greater on noncrosslinked plasma clots than on clots which had been crosslinked by Factor XIIIa. Furthermore, the uptake of plasminogen to crosslink...

Plasmin is a potent extracellular protease specialized in the degradation of fibrin (fibrinolysis). Active plasmin is generated by proteolytic activation of the zymogen plasminogen (Plg) by urokinase-type plasminogen activator (uPA) and tissue-type plasminogen activator (tPA). Alpha-enolase, although traditionally considered a glycolytic enzyme, constitutes a receptor for plasminogen on several...

A new fluorogenic peptide substrate for plasmin, 7-(N-succinoylalanylphenylalanyl-lysylamido)-4-methylcoumarin trifluoroacetate salt, was prepared that can be used in a simple and direct assay. The results obtained by the assay method are linear over a wide range of enzyme concentrations and sensitive enough to detect as little as 10(-5) CTA units of plasmin. By making use of the inhibitor Tras...

Background and aimsThe composition enzymology of human milk changes throughout the lactation period, differ for mothers who give birth prematurely compared to those deliver at full-term. Understanding from very low weight premature infants is great significance, objective this study was evaluate composition, protein profile plasmin activity delivered different gestational ages.MethodsSamples we...