background: activated platelets shed microparticles (mps) in vivo and certainly in vitro under storage. like platelets, platelet-derived mps contribute to hemostatic and inflammatory responses. we sought to determine the interactions between platelet mps and peripheral b lymphocytes in the healthy blood circulation to propose a possible role for platelet mps in the functioning of b cells.   mat...

BACKGROUND Therapeutic storage of platelet concentrate is a challenging problem for Transfusion Medicine, so that many studies have been carried out with the aim of improving the duration of storage of platelet concentrates. Little attention, however, has been given to the most appropriate biochemical methods for evaluating the quality of the stored platelet concentrates. MATERIAL AND METHODS...

The complex mechanism of altered platelet function during cardiopulmonary bypass (CPB) is a subject under investigation because it might result in understanding of blood-biomaterial interaction and, subsequently, optimal intervention. However, the common biochemical and functional parameters of platelets do not clarify the mechanism.’ Recently, new apparatuses were constructed to mimic the hemo...

Abstract Background Autologus platelet gel is easy to prepare and is relatively low cost. The aim of this study was to prepare and evaluate in vitro efficacy of autologus platelet gel. Materials and Methods In this experimental study, platelet concentrate and platelet poor plasma were prepared with aphaeresis method. Thrombin was prepared by mixing the plasma with calcium. Thrombin activity...

In the present study, we propose a theoretical framework to predict the recovery rates of platelets and white blood cells in the process of centrifugal separation of whole blood contained in a tube for the preparation of platelet-rich plasma. Compared to previous efforts to optimize or standardize the protocols of centrifugation, we try to further the physical background (i.e., based on the mul...

Reticulated platelets are immature platelets circulating in blood; they reflect the activity of megakaryopoiesis in the bone marrow. Therefore, they can be used as a non-invasive test in patients with thrombocytopenia in various clinical conditions. The preferred method of analysis is by flow cytometry. However, there is an evident lack of analytical standardization, making it difficult to comp...

Cigarette smokers, but not former smokers, excrete more thromboxane A2 (TxA2) metabolites in the urine than do lifelong nonsmokers, which suggests chronic activation of their platelets. To further characterize the effect elicited by smoking on platelet function, we followed the change in urinary excretion of the 2,3-dinor (Tx-M) and 11-dehydro (dTx) metabolites of TxA2, analyzed by gas chromato...

The clinical course of heparin-induced thrombocytopenia (HIT) may be separated into five sequential phases: 1. suspected HIT, 2. acute HIT, 3. subacute HIT A, 4. subacute HIT B, and 5. remote HIT. Each phase confronts the clinician with a unique set of management questions. In this review, the phases of HIT are defined and key management questions associated with each phase are discussed. Among...

This paper is a preliminary report of in vitro studies comparing platelet storage in the new polyolefin PL732 packs with the present polyvinyl PL146 packs. The parameters used to assess platelet viability in these studies were the recovery from the hypotonic stress test (HST) and the pH. The effect of the mass of concentrate was assessed by preparing 20 g, 35 g and 50 g packs. The method of agi...

OBJECTIVES To present a case of heparin induced thrombocytopenia in a patient treated with enoxaparin. METHODS A case of heparin-induced thrombocytopenia was examinated with a detailed platelet count analysis over the time and with detection of platelets antibodies. RESULTS The detection of platelet antobodies and the recovery of platelet count after cessation of enoxaparin strongly support...