D-(-)-3-Hydroxybutyrate (DHB), the immediate depolymerization product of the intracellular carbon store poly-3-hydroxybutyrate (PHB), is oxidized by the enzyme 3-hydroxybutyrate dehydrogenase to acetoacetate (AA) in the PHB degradation pathway. Externally supplied DHB can serve as a sole source of carbon and energy to support the growth of Sinorhizobium meliloti. In contrast, wild-type S. melil...

Genes encoding 3-hydroxybutyrate oligomer hydrolase (PhaZc) and 3-hydroxybutyrate dehydrogenase (Hbd) were isolated from Paracoccus denitrificans. PhaZc and Hbd were overproduced as His-tagged proteins in Escherichia coli and purified by affinity and gel filtration chromatography. Purified His-tagged proteins had molecular masses of 31 kDa and 120 kDa (a tetramer of 29-kDa subunits). The His-ta...

Plastic prepared from formulations of cornstarch and poly(b-hydroxybutyrate-co-b-hydroxyvalerate) (PHBV) biodegraded in tropical coastal waters. Biodegradation was monitored for 1 year. Starch—PHBV bioplastic appeared to lose weight in two overlapping phases until both biopolymers were entirely consumed. To examine the underlying degradation of starch and PHBV from biphasic weight-loss profiles...

A strain of Bacillus pumilus BP-171 with the ability heterotrophic nitrification-aerobic denitrification was isolated from a shrimp culture pond and showed good under laboratory conditions. In order to investigate effects its combinations different carbon sources, i.e., poly-3-hydroxybutyrate-co-3-hydroxyvalerate (PHBV) molasses, on growth performance shrimp, water quality bacterial community i...

1.Elliott, B. A., and Wilkinson, J. H., Serum “a-hydroxybutyrate dehydrogenase” in myocardial infarction and in liver disease. Lancet i, 698(1961). 2. Elliott, B. A., Jepson, E. M., and Wilkinson, J. H., Serum ahydroxybutyrate dehydrogenase-a new testwith improved specificity for myocardial lesions. Gun. Sci. 23, 305(1962). 3. Coodley, E. L., Evaluation of enzyme diagnosis in myocardial infarct...

This study discusses poly(3-hydroxybutyrate-co-4-hydroxybutyrate) that is produced by Delftia acidovorans bacteria in fed-batch cultures from various carbon sources. The experimentally determined optimal fed-batch culture conditions are as follows: pH: 7.0, incubation temperature: 26 8C, incubation time: 72 h, and concentration of carbon source: 10 g/L. The maximum 4HB unit composition of P(3HB...