CoFe nanowires have been synthesized by the electrodeposition technique into the pores of a polycarbonate membrane with a nominal pore diameter of 50 nm, and the composition of CoFe nanowires varying by changing the source concentration of iron. The synthesized nanowire surfaces were functionalized with amine groups by treatment with aminopropyltriethoxysilane (APTES) linker, and then conjugate...

Scanning electrochemical microscopy (SECM) was used to study horseradish peroxidase (HRP) immobilized with copolymer on insulating substrates (glass slide or polycarbonate membrane filter). Two methods were used to immobilize HRP: In the first, HRP was coimmobilized by cross-linking on a glass slide with a copolymer swelled in water to form a hydrogel; in the second, the same copolymer and avid...

A simple method for bonding polycarbonate, based on controlled exposure of the pieces to vapours of solvents, yields a tight seal and unmodified geometry of the channels.

Our previous studies on solute drag on frog skin and synthetic heteropore membranes have been extended to a synthetic homopore membrane. The 150-A radius pores of this membrane are formed by irradiation and etching of polycarbonate films. The membrane is 6-microm thick and it has 6 x 10(8) pores cm(-2). In this study, sucrose has been used as the driver solute with bulk flow blocked by hydrosta...

A procedure combining enrichment surface adhesion and polymerase chain detection (SA-PCR) was developed and applied in the detection of Listeria monocytogenes in meat products. Minced beef samples were inoculated with L. monocytogenes (log(10)3 cfu g(-1)) and incubated for 10 h at 30 degrees C in buffered peptone water. L. monocytogenes was recovered from the culture by attachment to a polycarb...

Shlyonsky V. Ion permeability of artificial membranes evaluated by diffusion potential and electrical resistance measurements. Adv Physiol Educ 37: 392–400, 2013; doi:10.1152/advan.00068.2013.—In the present article, a novel model of artificial membranes that provides efficient assistance in teaching the origins of diffusion potentials is proposed. These membranes are made of polycarbonate filt...

The lung epithelium has recently been identified as a novel site of fibrinogen (FBG) biosynthesis. A coordinated upregulation of A alpha, B beta, and gamma chain FBG gene transcription occurs upon stimulation of A549 lung epithelial cells with dexamethasone (DEX) and the proinflammatory mediator interleukin-6 (IL-6). Subsequently, the cells synthesize and secrete fully assembled FBG. This study...