نتایج جستجو برای: polyclonal antibodies
تعداد نتایج: 166975 فیلتر نتایج به سال:
background: the use of igys in a variety of methods in different areas of research, diagnostics, medical application and biotechnology should be considered widely. objectives: development of antibodies against extra cellular domain of influenza m2 (m2e) protein in egg yolk of laying hens. methods: a fusion construct harboring c-terminal of bovine heat shock protein 70 (hsp70) and influenza m2e ...
Background and Objective: Pseudomonas aeruginosa is an opportunistic pathogen that causes severe and lethal infections in immunocompromised individuals. This bacterium possesses a single polar flagellum. Flagellum and its subunit Flagellin play important roles in the pathogenesis of P. aeruginosa. Flagellin induces immune responses by interaction of its N-terminal domain with TLR-5. Our main ai...
Background: Regarding the importance of rapid detection of cholera toxin in environmental samples, an immunoassay method was developed for direct detection of cytotoxin B. Materials and Methods: The gene sequence of ctxB was cloned to pET28a vector and the recombinant protein was expressed in BL21 (DE3). The recombinant CtxB was purified by affinity chromatography and then used for immunizati...
We have performed a systematic variability study of polyclonal antibody catalysis by using five rabbits immunized with the same hapten. Important results from this work are the following. (1) Similarities were observed in the catalytic polyclonal antibodies derived from all five rabbits. Four of the five rabbits produced polyclonal samples that were nearly the same in terms of catalytic activit...
BACKGROUND & OBJECTIVES Estimation of parathyroid hormone (PTH) levels is important in the management of metabolic bone disorders. Here we describe a simple, sensitive and specific second generation immunoradiometric assay (IRMA) to detect intact PTH levels using different solid phase matrices. Different methods for immobilization of antibodies have also been evaluated. METHODS Experiments we...
Kresoxim-methyl was one of the two first strobilurins to be discovered, and nowadays it is widely used as an antifungal agent in crop protection. Because of its low molecular weight and negligible structural complexity, the generation of antibodies to kresoxim-methyl noticeably requires the preparation of functionalized haptens. In this study, the introduction of a hydrocarbon spacer arm at the...
Polyclonal antibodies against the Hop1 phospho-T318 and phospho-S298 were obtained as following: The α-pT318 polyclonal antibody [Cambridge Research Biochemicals] was obtained by immunising two rabbits with the antigenic [C]-Ahx-ASIQP-[pT]-QFVSN where C is a cysteine residue added at the N-terminus of the peptide, Ahx is aminohexanoicacid and pT is a phosphorylated threonine residue. Upon bleed...
Circulating inhibitors against von Willebrand factor (vWF) that show the properties of heterologous IgG antibodies have been described in a few patients with severe von Willebrand disease (vWD). The present study provides further characterization of inhibitors from two patients with severe vWD. Inhibitors in both, like polyclonal rabbit antibody, detected all sizes of multimers and the complex ...
An important issue for the performance and specificity of an antibody is the nature of the binding to its protein target, including if the recognition involves linear or conformational epitopes. Here, we dissect polyclonal sera by creating epitope-specific antibody fractions using a combination of epitope mapping and an affinity capture approach involving both synthesized peptides and recombina...
Quantitative data are presented on (i) the intertypic cross-reactions of polyclonal, guinea pig antibodies directed against the N or H antigen of type 1 poliovirus and (ii) a set of five nonneutralizing, mouse hybridoma antibodies raised against N antigen or a mixture of capsid polypeptides VP1, VP2, and VP3. Three of these antibodies recognize H antigen and VP1, the fourth H antigen only, and ...
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