A method is described for the rapid and efficient isolation of phagocytic vesicles from large scale cultures of Acanthamoeba castellanii (Neff) that have been incubated with polystyrene latex beads. Cells were allowed to phagocytose latex beads for 30 min and then were homogenized, and the phagocytic vesicles were isolated by one centrifugation through several layers of sucrose . Identity and p...

Realisation of a device intended for the manipulation and detection of bead-tagged DNA and other bio-molecules is presented. Acoustic radiation forces are used to manipulate polystyrene micro-beads into an optical evanescent field generated by a laser pumped ion-exchanged waveguide. The evanescent field only excites fluorophores brought within approximately 100 nm of the waveguide, allowing the...

We present a novel device employing one million femtoliter droplets immobilized on a substrate for the quantitative detection of extremely low concentrations of biomolecules in a sample. Surface-modified polystyrene beads carrying either zero or a single biomolecule-reporter enzyme complex are efficiently isolated into femtoliter droplets formed on hydrophilic-in-hydrophobic surfaces. Using a c...

In this work, we are motivated by the need to produce particles of well-controlled size, shape and morphology for general application in catalysis, environmental remediation, nanomedicine, pharmaceuticals, the development of new materials, and other fields. Moreover, our approaches are guided by the desire for continuous and scalable production, in contrast to the batch-wise processes typically...

Actin polymerization is required for the generation of motile force at the leading edge of both lamellipodia and filopodia and also at the surface of motile intracellular bacterial pathogens such as Listeria monocytogenes. Local catalysis of actin filament polymerization is accomplished in L. monocytogenes by the bacterial protein ActA. Polystyrene beads coated with purified ActA protein can un...

The integrity of tunnel structures deployed in difficult geological conditions such as expansive soils can be improved by incorporating compressible layers the lining. Such lining materials obtained embedding weak inclusions (Expanded polystyrene, Expanded glass beads, Air voids) into a cement paste material. material properties these individual constituents well their geometry affect overall b...

Increasing requirements are leading to new developments in bead foam materials. Engineering thermoplastics such as polybutylene terephthalate (PBT) outperform standard foams thermal stability. In order obtain molded parts, beads fused together using steam chest molding. Classical theories for the fusion mechanism, explaining molding of amorphous EPS (expandable polystyrene) or semi-crystalline ...

BACKGROUND Cytokines and chemokines are relevant biomarkers of pathology and immunity to infectious diseases such as malaria. Several commercially available kits based on quantitative suspension array technologies allow the profiling of multiple cytokines and chemokines in small volumes of sample. However, kits are being continuously improved and information on their performance is lacking. M...

In this work, the implementation of a femtosecond Stimulated Raman Scattering microscope, equipped with three laser sources: Titanium-Sapphire (Ti:Sa), an optical parametric oscillator (OPO), and second harmonic generator (SHG); is presented. Our microscope designed so that it can cover all regions spectra, taking advantage two possible combinations. The first, Ti:Sa OPO beams, which C-H region...

The CellJet microfluidic dispenser chip prints single living cells encapsulated in free-flying droplets. Two sets of parallel facing electrodes in a 50 x 55 μm channel are applied to measure the presence and velocity of a single cell in real-time. Typically a 500 pl droplet is printed on demand, when a cell is at the nozzle. Feeding 20 μm polystyrene beads, a cell model, resulted in a peak-to-p...