The synthesis and structural characterisation of a novel C3-symmetric tris-bidentate ligand, L, featuring a triphenylamine core appended by pyridylimine coordination sites is reported: 1H NMR compleximetric titration studies with Ag(I) and ESMS indicate the presence of [Ag3L2]3+ species in solution, consistent with the formation of a trinuclear double helicate complex: the Trinity helix.

The capabilities of contemporary differential scanning and isothermal titration microcalorimetry for studying the thermodynamics of protein unfolding/refolding and their association with partners, particularly target DNA duplexes, are considered. It is shown that the predenaturational changes of proteins must not be ignored in studying the thermodynamics of formation of their native structure a...

Positron annihilation Dopplerbroadening measurements were carried out on Cu-Al-Ni based alloys to which Ti, and Ti and Mn was added. The measurements reveal a drastic effect in the S-parameter behavior when the defects anneal out at the transition from martensite to the beta phase. When crossing the transition region several times, an increase of the S-parameter value is observed. This is inter...

The author corroborates the data of Schmidt showing that the dissociation index of the third group of arginine is pK(3)' = 12.5. New titration data of edestin have been obtained in very alkaline solutions and show that there is a corresponding group with a titration index of pG' = 12.0, but present in much less quantity than can account for the arginine found on hydrolysis. The data support the...

Both rat liver and Escherichia coli rRNA in 0.1M-sodium chloride were titrated with acid or alkali over the range pH 3-7 at approx. 0°C. rRNA did not bind acid reversibly and hysteresis was observed, i.e. the plot of acid bound to rRNA against pH had the form of a loop showing that the amount of acid bound at a particular pH depended on the direction of the titration. Although the boundary curv...

We developed a rapid method for estimating the amyloid beta (Aβ)-conformation state related with Alzheimer's disease. We prepared gold nanoparticle (AuNP)-Aβ antibody conjugates treated with bovine serum albumin to stabilize their dispersibility in a buffer. The prepared AuNPs were precipitated in the presence of Aβ aggregates, such as oligomers and fibrils. Aβ monomers did not precipitate AuNP...

CLINICAL CHEMISTRY, Vol. 27, No. 1, 1981 197 the use of this reagent or the commercial Beckman reagent, showed no significant bias. In addition, we observed no discrepancies in our quality control pool determinations or ADC numbers. We conclude that this creatinine reagent performs well and that the added diethylamine provides a simple and effective means of preventing precipitate formation for...

Formation and dynamics of an Al(OH)3 precipitation ring is studied by diffusing NaOH into a gel containing AlCl3. Limited feeding of the outer electrolyte (NaOH) is found to yield an intricate ring-dynamics which involves stopping and reversal of the direction of motion of the precipitation ring, and evolution into stationary multi-ring structures. A model of the ring-dynamics is developed by c...

Aerobic sludge digestion in the presence of Fenton's reagent and hydrogen peroxide was examined. Fenton's reagent considerably improved the aerobic digestion process in comparison with hydrogen peroxide as well as classical aerobic digestion. Application of Fenton's reagent also led to improvement of settling and dewatering properties of sludge and much better clarity of supernatant. Its drawba...

It is extremely rare that the urinary bladder stones cause vesicorectal fistulas. Urinary tract infection and poor fluid intake are the main etiologies that precipitate vesical stone formation, and subsequent rectovesical fistula. We present a complicated case of neglected vesical stone, which recurred 3 times. The patient presented with passage of urine per rectum leading to fecal incontinence...