Recent research suggested a metabolic implication of osteocalcin (OCN) in e.g. insulin sensitivity or steroid production. We used an untargeted metabolomics approach by analyzing plasma and urine samples of 931 participants using mass spectrometry to reveal further metabolic actions of OCN. Several detected relations between OCN and metabolites were strongly linked to renal function, however, a...

The Gram-positive bacterium Bacillus subtilis encounters nutrient limitations and osmotic stress in its natural soil ecosystem. To ensure survival and sustain growth, highly integrated adaptive responses are required. Here, we investigated the system-wide response of B. subtilis to different, simultaneously imposed stresses. To address the anticipated complexity of the cellular response network...

In order to investigate the physiological effects of exogenous γ-aminobutyric acid (GABA) on drought tolerance in white clover (Trifolium repens), GABA shunt, polyamines (PAs), and proline (Pro) metabolism were examined after plants pretreated with or without GABA (8 mM) and then exposed to water or 15% PEG-induced drought stress in growth chamber. In this study, exogenous application of GABA e...

In mammals, L-arginine is classified as a semiessential or conditionally essential amino acid, depending on the developmental stage and health status of the individual. It can be derived from proline or glutamate, with the ultimate synthetic step catalyzed by argininosuccinate lyase. L-arginine is catabolized by arginases, nitric oxide synthases, arginine:glycine amidinotransferase, and possibl...

This study investigated how salicylic acid (SA) mediates the response of melon (Cucumis melo) seeds to salt stress using physiological and transcriptomic methods. The effects SA on antioxidant enzymes, osmoregulatory substances, transcriptome under were sodium chloride (NaCl, 100 mmol·L−1) as stimulant + NaCl (0.25 mmol·L−1 alleviation treatment. results showed that positively influences tolera...

Arginine, a semiessential or conditionally essential amino acid in humans, is one of the most metabolically versatile amino acids and serves as a precursor for the synthesis of urea, nitric oxide, polyamines, proline, glutamate, creatine, and agmatine. Arginine is metabolized through a complex and highly regulated set of pathways that remain incompletely understood at both the whole-body and th...

Tasidotin, an oncolytic drug in phase II clinical trials, is a peptide analog of the antimitotic depsipeptide dolastatin 15. In tasidotin, the carboxyl-terminal ester group of dolastatin 15 has been replaced by a carboxy-terminal tert-butyl amide. As expected from studies with cemadotin, [(3)H]tasidotin, with the radiolabel in the second proline residue, was hydrolyzed intracellularly, with for...

introduction plant responses to environmental stress have a central role in agricultural production. responses results from events occurring at all levels of the organization, from biochemical reactions in cells to whole plant physiology. many plants are injured when exposed to low non-freezing temperatures. however, data on the effects of night temperatures are scarce. on the other hand, nitri...

A study has been made of the distribution and metabolism of protein-bound hydroxyproline in an elongating tissue, the excised Avena coleoptile. The hydroxyproline-containing proteins of this tissue have been separated into 3 fractions on the basis of their solubilities. The cytoplasmic, trichloroacetic acid-insoluble proteins (S-fraction) contain the bulk of the proline of the cells but only 20...

Proline is a readily released stress substrate that can bemetabolized by proline oxidase (POX) to generate either reactive oxygen species (ROS) to induce apoptosis or autophagy or ATP during times of nutrient stress. However, the contribution of prolinemetabolism to tumorigenesis in hypoxicmicroenvironments has not been explored. In this study, we investigated the different functions of POX und...