Materials/Method: To examine the relationship between BIM protein expression and genomic BIM loss, we performed Western blotting analysis using whole cell lysates of the seven MCL cell lines. This expression was compared to the results of array CGH, especially for the relationship with BCL2, the antagonist of BIM. In order to understand the relevance of apoptosis-related genes in MCL, published...

conclusions in the present study, we identified a key gene rps16 and a significant module involved in ribosome biogenesis in eukaryotes that were related to neonatal sepsis, which might be potential biomarkers for early detection and therapy for neonatal sepsis. background the exact interacting factor that response to the infection for neonatal sepsis is still needed to urgently to be disclosed...

Numerical design optimization is a powerful tool that can be used by engineers during any stage of the design process. There are many different applications for structural optimization. A specific application that will be discussed in the following paper is experimental data matching. Data obtained through tests on a physical structure will be matched with data from a numerical model of that sa...

We extend our previously-derived generalized closed-form representation for spectral dispersing performance of the VirtuallyImaged-Phased-Array (VIPA) based on a 3D vectorial Gaussian beam formulation to demultiplexing application. To analyze VIPA in the demultiplexer scheme, a spherical lens is added after the VIPA, so that the device plane is superimposed on the focal plane of the lens. The c...

PURPOSE To adapt membrane-bound antibody array (MA) technology to characterize the distribution of a wide range of bioactive trace proteins in reflex (RTF) and open-eye (OTF) and closed-eye (CTF) tear samples. METHODS Tears were collected by capillary tube and centrifuged. A commercially available standard MA and a custom array were modified to maximize the sensitivity of detection and the si...

We investigated characteristics of germline copy number variations (CNV) in BRCA1-associated ovarian cancer patients by comparing them to CNVs present in sporadic ovarian cancer patients. Germline CNVs in 51 BRCA1-associated, 33 sporadic ovarian cancer patients, and 47 healthy women were analyzed by both signal intensity and genotyping data using the Affymetrix Genome-Wide Human SNP Array 6.0. ...

To detect interactions between proteins of vaccinia virus, we carried out a comprehensive two-hybrid analysis to assay every pairwise combination. We constructed an array of yeast transformants that contained each of the 266 predicted viral ORFs as Gal4 activation domain hybrid proteins. The array was individually mated to transformants containing each ORF as a Gal4-DNA-binding domain hybrid, a...

The success of genome-wide association studies (GWASs) has enabled us to improve risk assessment and provide novel genetic variants for diagnosis, prevention, and treatment. However, most variants discovered by GWASs have been reported to have very small effect sizes on complex human diseases, which has been a big hurdle in building risk prediction models. Recently, many statistical approaches ...

An electrochemical impedance biosensor array with protein-modified electrodes was designed and fabricated in this report. To demonstrate its feasibility of the detection of multiple antigen-antibody binding reactions based on a label-free approach, human IgG (hIgG), rat IgG (rIgG), human globin and bovine serum albumin were immobilized, respectively, on the gold electrodes and then the resultan...

A novel ergodic cellular automaton model of gene-protein network is presented. It shown that the presented can predict occurrences typical nonlinear phenomena a conventional ordinary differential equation model. In addition, theoretical analysis methods are proposed. Using methods, an important advantage revealed: better suited to compared regular (standard) automaton. Furthermore, implemented ...