background: radiation-induced molecular changes on the endothelial surface of brain arteriovenous malformations (avm) may be used as markers for specific vascular targeting agents. in this study, we examined the level of expression of platelet endothelial cell adhesion molecule-1 (pecam-1) on brain endothelial cell surface after radiation treatment, with the aim of targeting the radiation-induc...

background and objectives: during past decades hansenula polymorpha has attracted global attention for the expression of recombinant proteins due to its high growth rate, minimal nutritional porequirements and use of methanol as a low cost inducer. materials and methods: the corresponding nucleotide sequences for the expression of heterologous genes in hansenula poylmorpha were extracted and as...

background: herpes simplex virus type 2 (hsv-2) is highly prevalent and major cause of genital herpes in humans. the life-long nature of infection and the increasing prevalence of genital herpes imply that vaccination is the best strategy for controlling the spread of infection and limiting hsv disease. hsv glycoprotein d (gd) is one of the most important viral immunogen which has an essential ...

fusion of two genes at dna level produces a single protein, known as a chimeric protein. immunotoxins are chimeric proteins composed of specific cell targeting and cell killing moieties. bacterial or plant toxins are commonly used as the killing moieties of the chimeric immunotoxins. in this investigation, the catalytic domain of shiga-like toxin (a1) was fused to human granulocyte macrophage c...

a new strategy for construction of synthetic gene encoding human basic fibroblast growth factor comprising dna annealing-ligation and augmentation by polymerase chain reaction was introduced. the sequence of the gene and corresponding amino acid chain were modified in order to increase stability of the protein. first, 300 bp and 160 bp fragments of the gene were assembled from 18 oligonucleotid...

background: cholera toxin b subunit (ctb) has been extensively considered as an immunogenic and adjuvant protein, but its yield of expression is not satisfactory in many studies. the aim of this study was to compare the expression of native and mutant recombinant ctb (rctb) in pqe vector. methods: ctxb fragment from vibrio cholerae o1 atcc14035 containing the substitution of mutant ctxb for ami...

with advent and development of dna recombinant technology and advantages of p. pastoris expression system, fusion (f) protein of pprv expression, because of effective immunodominant role could be an appropriate candidate for production of recombinant vaccine against ppr disease. in this study, f gene of pprv nigeria 75/1 strain (1637 bp) was amplified using rt-pcr and purified. it was then clon...

introduction: the development of an effective vaccine against hepatitis c virus (hcv) is still a target of intensive vaccine research. the hcv envelope proteins e1 and e2 which can induce broadly neutralizing antibodies are the major candidate for this purpose. different types of expression systems have been used to express these glycoproteins. in this study, an expression system using pichia p...