Ubiquitination relies on a subtle balance between selectivity and promiscuity achieved through specific interactions between ubiquitin-conjugating enzymes (E2s) and ubiquitin ligases (E3s). Here, we report how a single aspartic to glutamic acid substitution acts as a dynamic switch to tip the selectivity balance of human E2s for interaction toward E3 RING-finger domains. By combining molecular ...

Altered growth factor responses in phospho-protein-driven signaling networks are crucial to cancer cell survival and pathology. Profiles of cancer cell signaling networks might therefore identify mechanisms by which such cells interpret environmental cues for continued growth. Using multiparameter flow cytometry, we monitored phospho-protein responses to environmental cues in acute myeloid leuk...

parkinson’s disease (pd) is a progressive neurological disorder characterized by tremor, rigidity, and slowness of movements. specific changes associated with various pathological attacks in parkinson’s disease can be indicated in causality interactions of the brain network from resting state fmri data. in this paper, we aimed to reveal the network architecture of the directed influence brain n...

Background: Prediction of the protein localization is among the most important issues in the bioinformatics that is used for the prediction of the proteins in the cells and organelles such as mitochondria. In this study, several machine learning algorithms are applied for the prediction of the intracellular protein locations. These algorithms use the features extracted from pro...

conclusions our experimental and in silico findings may provide new insight in the complex molecular pathways of drug resistance and also could assist design an efficient therapeutic strategy for aspergillosis treatment. results four out of five isolates revealed cyp51a and mdr1 mrna overexpression. interestingly, the isolate, which was negative for cyp51a and mdr1 mrna expression showed a high...

Gene identification represents the first step to a better understanding of the physiological role of the underlying protein and disease pathways, which in turn serves as a starting point for developing therapeutic interventions. Familial hypercholesterolemia is a hereditary metabolic disorder characterized by high low-density lipoprotein cholesterol levels. Hypercholesterolemia is a quantitativ...

Hematite (α-Fe2O3) nanorods were synthesized by hydrothermal method using Cetyltrimethylammonium bromide (CTAB) as a surfactant agent. To study optical, nanostructural properties, and to control the morphology and shape of nanorods, 0.025 mol L-1, 0.05 mol L-1 and 0.1 mol L-1 concentration of CTAB were used. Moreover, the effect of interaction between bovine serum albumin (BSA) A9418-5G protein...