Mechanisms by which microtubule plus ends interact with regions of cell-cell contact during tissue development and morphogenesis are not fully understood. We characterize a previously unreported interaction between the microtubule binding protein end-binding 1 (EB1) and the desmosomal protein desmoplakin (DP), and demonstrate that DP-EB1 interactions enable DP to modify microtubule organization...

Microtubule plus-end tracking proteins are crucial for the regulation of microtubule dynamics. Preitner et al. report that one such protein, adenomatous polyposis coli (APC), also binds RNA and identify mRNAs encoding tubulin subunits within the brain APC-RNA interactome, suggesting a new mode of microtubule self-regulation.

Prostate cancer (PC) is one of the prevalent tumors in men causing higher mortality. Luteoloside has been discovered for suppressive role into progression some cancers. However, Luteoloside’s regulatory functions regarding PC are not well understood. This study thus designed to investigate impact on progression. In this work, it was demonstrated that cell proliferation gradually inhibited with ...

Endometrial cancer (EC) is one of the three main gynecological cancers. Identifying new therapeutic targets and further elucidating molecular mechanisms EC tumorigenesis have important implications for women’s health. The Go-Ichi-Ni-San (GINS) family, which includes four subunits (GINS1–4), has specific functions in DNA replication cell cycle. Cancer Genome Atlas (TCGA) data showed that GINS2 t...

Design and synthesis of pseudo-natural products (PNPs) through recombination natural product (NP) fragments in unprecedented arrangements enables the discovery novel biologically relevant chemical matter. With a view to wider coverage NP-inspired biological space, we describe combination this principle with macrocycle formation. PNP-macrocycles were synthesized efficiently stereoselective one-p...

One mechanism for the reappearance of G protein-coupled receptors after agonist activation is microtubule-based transport. In pressure-overload cardiac hypertrophy, there is downregulation of G protein-coupled receptors and the appearance of a densified microtubule network extensively decorated by a microtubule-associated protein, MAP 4. Our hypothesis is that overdecoration of a dense microtub...

Several microtubule binding proteins, including CLIP-170 (cytoplasmic linker protein-170), CLIP-115, and EB1 (end-binding protein 1), have been shown to associate specifically with the ends of growing microtubules in non-neuronal cells, thereby regulating microtubule dynamics and the binding of microtubules to protein complexes, organelles, and membranes. When fused to GFP (green fluorescent pr...

The centrosome is the main microtubule organising centre in the cell. During mitosis, centrosomes dramatically increase microtubule nucleating activity, enabling them to form a mitotic spindle. Recent studies show that Aurora A kinase promotes microtubule assembly from centrosomes through the phosphorylation of the conserved centrosomal protein TACC.