in this paper detection of endoglucanase gene and protein profile belonging to two fungal species, penicillium oxalicum 1sms and trichodermaviride156ms with high cellulotic activity was investigated. fungi isolates were cultured on inducer cmc medium and then released sugar and protein were assayed every three days for a month using arsenate molybdate reagent and bradford method, respectively. ...

MOTIVATION The observed correlations between pairs of homologous protein sequences are typically explained in terms of a Markovian dynamic of amino acid substitution. This model assumes that every location on the protein sequence has the same background distribution of amino acids, an assumption that is incompatible with the observed heterogeneity of protein amino acid profiles and with the suc...

Electrophoresis is a meticulous method in para-clinical science but has received less attention. In this study, the protein profile of synovial fluid of carpal joint and blood serum in buffalo was detected. Sampling was performed by arthrocentesis of 80 left and right forelimbs and obtaining blood serums of 40 Bubalus bubalis in Ahvaz industrial slaughterhouse. Samples were str...

The expression of endoglucanase gene and protein profile belonging to two fungal species, Penicillium oxalicum 1SMS and Trichoderma viride 156MS with high cellulase enzyme activity, was investigated. Fungal isolates were cultured on inducer CMC medium and then the amount of released sugar and protein were assayed every three days for a month, using arsenate molybdatereagent and Bradford method,...

Background: Culture media enrichment through the addition of protein hydrolysates is beneficial for achieving higher protein expression. Methods: In this study, designing the optimum mixture of four soy and casein-derived hydrolysates was successfully performed by design of experiment and specific productivity increased in all predicted combinations. Protein profile of recombinant CHO (rCHO) ce...

MOTIVATION Protein homology detection and sequence alignment are at the basis of protein structure prediction, function prediction and evolution. RESULTS We have generalized the alignment of protein sequences with a profile hidden Markov model (HMM) to the case of pairwise alignment of profile HMMs. We present a method for detecting distant homologous relationships between proteins based on t...

The application of the mean force field in protein mutant stability prediction is explored. Based on protein main chain characteristics, including polar fraction, accessibility and dihedral angles, the mean force field was constructed to evaluate the compatibility between an amino acid residue and its environment, from which a position-dependent protein mutant profile was constructed. At each p...

Experimentally determined protein tertiary structures are currently increasing in an enormous rate. The growth of the structure database makes structure prediction methods based on known tertiary structures more efficient. Detecting weak homology is crucial to extend applicable area of these methods. For this purpose, a profile or PSSM (position-specific score matrix) derived from multiple alig...

Abstract Liver is a by-product that can be used as raw material for nuggets because it has the nutrients needed growth especially children. Processing liver into diversification effort. Different flours will produce different final products. The purpose of this research was to determine chemical quality and amino acid profile with filler agents. materials were fresh chicken beef liver, cornstar...