PURPOSE The turnover of chick scleral proteoglycans from control and form-vision deprived (myopic) eyes was compared in vivo and in explant cultures to determine whether proteoglycan degradation is altered during the development of myopia and to characterize the mechanism of proteoglycan turnover in the sclera. METHODS Seven-day-old chicks were radiolabeled via an intraperitoneal injection of...

Proteoglycans, proteins, and glycoproteins were extracted from femoral condyle (FC) and metacarpalphalangeal (MP) articular cartilages and fractionated by sequential density gradient sedimentation under dissociative conditions. The major high density proteoglycan (PG-I) accounted for 74% of the total proteoglycan extracted from the MP cartilage and 79% of that from the femoral condyle. These pr...

Infectious diseases remain an important global health problem. The interaction of a wide range of pathogen bacteria with host cells from many different tissues is frequently mediated by proteoglycans. These compounds are ubiquitous complex molecules which are not only involved in adherence and colonization, but can also participate in other steps of pathogenesis. To overcome the problem of micr...

Proteoglycans have been extracted from rabbit corneal stromas at developmental stages from fetal to adult. Ion exchange fractionation and gel chromatography show that proteodermatan sulfates decrease in sulfation and relative amount and proteokeratan sulfates increase in sulfation and relative amount during development. There are small increases in size of all of the proteoglycans up to 2 weeks...

Muscle fiber number is determined around the time hatch with continued posthatch muscle growth being mediated by the adult myoblast, satellite cell, population of cells. Satellite cells are dynamic in their expression of proteins including the cell membrane associated proteoglycans, syndecan-4 and glypican-1. These proteoglycans play roles in organizing the extracellular environment in the sate...

Heparan sulfate proteoglycans are ubiquitously found at the cell surface and extracellular matrix in all the animal species. This review will focus on the structural characteristics of the heparan sulfate proteoglycans related to protein interactions leading to cell signaling. The heparan sulfate chains due to their vast structural diversity are able to bind and interact with a wide variety of ...

In vitro studies have suggested that proteoglycans facilitate signaling by mammalian growth factors, but genetic evidence supporting this role has been lacking. Here, we characterize the ENU-induced mutation lazy mesoderm (lzme), which disrupts the single mouse gene encoding UDP-glucose dehydrogenase (Ugdh), an enzyme required for the synthesis of the glycosaminoglycan (GAG) side chains of prot...

action was the pluripotential haemopoietic stem cell (CFUS ) . Whenever they were tested, free glycosaminoglycan chains were unable to reproduce P-D-xyloside influences on cell behaviour. On the basis of the cited biochemical evidence it seems likely that the xyloside effects are mediated through alterations in surface membrane proteoglycans. Most probably the xyloside-induced changes follow fr...