information avalanche (overload or expansion) in various scientific fields is a novel issue turned out by a number of factors considered necessary to facilitate their record and registration. though, the biological science and its diverse fields like proteomics are not immune of this event and even may be as the event’s herald. on the other hand, time as the most valued anxiety of human has enc...

The methods of (14)C-2-deoxyglucose ((14)C-2DG) metabolic mapping and optical intrinsic signal (OIS) imaging were used to evaluate the response evoked in the contralateral primary somatosensory receiving areas (SI and SII) of anesthetized cats by either 25 Hz ("flutter") or 200 Hz ("vibration") sinusoidal vertical skin displacement stimulation of the central pad on the distal forepaw. Unilatera...

Mollicutes (mycoplasmas) are pathogenic in a wide range of mammals (including humans), reptiles, fish, arthropods, and plants. Of the medically important mollicutes, Mycoplasma gallisepticum is of particular relevance to avian agriculture and veterinary science, causing chronic respiratory disease in poultry and turkey. Using two-dimensional electrophoresis based quantitative expression proteom...

Introduction 2 Deoxyribonucleic Acid to Ribonucleic Acid 4 Ribonucleic Acid to Protein 6 Proteome Handling in Classical and Functional Proteomics 6 Protein Purification 6 Cell Culture 8 Other Source of Samples 8 Subpopulation of Cells 10 Subcellular Components 11 Protein Separation by Two-Dimensional Gel Electrophoresis 12 Principles of Two-Dimensional Gel Electrophoretic Separation 13 Detectio...

This Biomedicine Select explores a cluster of recent papers that highlight traditional and new approaches to treating a variety of diseases—from a small-molecule inhibitor to treat temporal lobe epilepsy to cell replacement therapy for diabetes to a unique way to generate tumor-specific antigens. Epilepsy affects more than 50 million people worldwide and about 40% of those with the disease cont...

In this work, we evaluate the incorporation of an ultralow flow interface for coupling capillary electrophoresis (CE) and mass spectrometry (MS), in combination with reversed-phase high-pressure liquid chromatography (HPLC) fractionation as an alternate workflow for quantitative proteomics. Proteins, extracted from a SILAC (stable isotope labeling by amino acids in cell culture) labeled and an ...

The pioneering work by Patrick H. O'Farrell established two-dimensional gel electrophoresis as one of the most important high-resolution protein separation techniques of modern biochemistry (Journal of Biological Chemistry1975, 250, 4007-4021). The application of two-dimensional gel electrophoresis has played a key role in the systematic identification and detailed characterization of the prote...