A major challenge in cell biology is to identify the subcellular distribution of proteins within cells and to characterize how protein localization changes under different cell growth conditions and in response to stress and other external signals. Protein localization is usually determined either by microscopy or by using cell fractionation combined with protein blotting techniques. Both these...

Alveolar macrophages (AM) belong to a phenotype of macrophages with distinct biological functions and important pathophysiological roles in lung health and disease. The molecular details determining AM differentiation from blood monocytes and AM roles in lung homeostasis are largely unknown. With the use of different technological platforms, advances in the field of proteomics have made it poss...

glaucoma, a group of multifactor ocular diseases, is the second leading cause of blindness worldwide. primary open angle (poa) is the most common type of glaucoma, characterized by progressive optic nerve degeneration. numerous genes and proteins have been revealed to be associated with poag, but the pathologic mechanisms of the disease are still poorly understood. proteomics, the collective st...

As the range of techniques for microwave heating has expanded, so have the areas in which it can have a profound impact. Two emerging areas are the application of microwave heating for the synthesis of peptides, peptoids, oligopeptides and carbohydrates and in the field of proteomics.

Personalized medicine is the most promising approaches in the treatment of various diseases, especially cancer. The use of appropriate biomarkers for personalized treatment has advantage over conventional therapeutics approach, as it confer maximum effectiveness with minimum side effect. Personalized treatment can be achieved by implementation of omic studies in clinical practices. Application ...

The European Bioinformatics Community for Mass Spectrometry (EuBIC-MS; eubic-ms.org) was founded in 2014 to unite computational mass spectrometry researchers and proteomics bioinformaticians working academia industry. EuBIC-MS maintains educational resources (proteomics-academy.org) organises workshops at national international conferences on spectrometry. Furthermore, is actively involved seve...

The development of high-speed analytical techniques such as next-generation sequencing and microarrays allows high-throughput analysis of biological information at a low cost. These techniques contribute to medical and bioscience advancements and provide new avenues for scientific research. Here, we outline a variety of new innovative techniques and discuss their use in omics research (e.g., ge...

Full professor of Analytical Chemistry at Piemonte Orientale University, Department of Environmental and Life Sciences. His scientific and research activity concerns on: develop, optimisation and validation of techniques, analytical, medical diagnostic optimisation, proteomics, certification of business and laboratory quality, characterisation of environmental systems, diagnostic of products an...

Peptidomics is an emerging field branching from proteomics that targets endogenously produced protein fragments. Endogenous peptides are often functional within the body-and can be both beneficial and detrimental. This review covers the use of peptidomics in understanding digestion, and identifying functional peptides and biomarkers. Various techniques for peptide and glycopeptide extraction, b...

Proteomics can be defined as the large-scale analysis of proteins. Due to the complexity of biological systems, it is required to concatenate various separation techniques prior to mass spectrometry. These techniques, dealing with proteins or peptides, can rely on chromatography or electrophoresis. In this review, the electrophoretic techniques are under scrutiny. Their principles are recalled,...