نتایج جستجو برای: protoplast fusion
تعداد نتایج: 120504 فیلتر نتایج به سال:
Sonicated unilamellar lipid vesicles, consisting of egg lecithin, stearylamine, and cholesterol in 7:2:1 molar ratios, promoted the aggregation of tobacco (Nicotiana glutinosa) protoplasts with the aid of mono- or divalent cations.A reaction mixture containing liposomes (0.4 micromoles lipid per milliliter), 50 millimolar CaCl(2), 0.5 molar mannitol, and 5 x 10(5) protoplasts per milliliter res...
The moss Physcomitrella patens is an ideal model plant to study plant developmental processes. To better understand the mechanism of protoplast regeneration, a phosphoproteome analysis was performed. Protoplasts were prepared from protonemata. By 4 d of protoplast regeneration, the first cell divisions had ensued. Through a highly selective titanium dioxide (TiO2)-based phosphopeptide enrichmen...
Tolulope Modupe Adeleye*, Sharafadeen Olateju Kareem, Bankole Mobolaji Olufunmilayo, Olusegun Atanda, Michael Bamitale Osho, and Olawale Dairo. Microbiol. Biotechnol. Lett. 2021;49:45-56. https://doi.org/10.48022/mbl.2003.03014
Abstract In the present study, a high yield of isolated protoplasts from agronomically important crop Fagopyrum esculentum was obtained by applying mixture cellulase, pectolyase, and driselase. We demonstrated that morphogenic callus-derived 1 × 10 6 per g fresh tissue. For hypocotyls used as protoplast source, number released cells twice lower. The protoplasts, embedded in an agarose matrix cu...
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