Four hundred and twenty-two beef cattle of two different breeds (purebred Hereford and composite multibreed) were characterized by polymerase chain reaction-restriction fragment length polymorphism, using the restriction enzymes ApaI, AvaII, HindIII, PstI, SpeI, SspI and TaqI in two regions (the D-loop and the ND-5 gene) of mitochondrial DNA. The association between molecular haplotypes and rec...

BglII 5’...A_GATCT...3’ (5’overhang) BamHI 5’...G_GATCC...3’ (5’overhang); notice: MboI and Sau3AI = 5’..._GATC...3’ EcoRI 5’...G_AATTC...3’ (5’ overhang) HpaII 5’...C_CGG...3’ (5’ overhang); notice: CpG methylation blocked HindIII 5’...A_AGCTT...3’ (5’ overhang) KpnI 5’...GGTAC_C...3’ (3’ overhang) NcoI 5’...C_CATGG...3’ (5’ overhang); notice: Kozak sequence (RCC)ACCATGG NotI 5’...GC_GGCCGC......

A 3,167-bp PstI fragment of genomic DNA from Pseudomonas sp. strain B13 was cloned and sequenced. The gene clcE consists of 1,059 nucleotides encoding a protein of 352 amino acids with a calculated mass of 37,769 Da which showed maleylacetate reductase activity. The protein had significant sequence similarities with the polypeptides encoded by tcbF of pP51 (59.4% identical positions), tfdF of p...

the present study was aimed to examine the association of bovine follicular stimulating hormone gene polymorphism with sperm quality traits including sperm volume (sv), sperm concentration (spco), total sperm (ts), fresh sperm motility (fsm), total fresh motile sperm (tfms), post thaw sperm motility (ptsm), total post thaw motile sperm (tptms), number of produced payout (npp), number of fresh m...

Human cytochrome P4502E1 (CYP2E1) is a well-conserved xenobiotic-metabolizing enzyme expressed in liver, kidney, nasal mucosa, brain, lung, and other tissues. CYP2E1 is inducible by ethanol, acetone, and other low-molecular weight substrates and may mediate development of chemically-mediated cancers. CYP2E1 polymorphisms alter the transcriptional activity of the gene. This study was conducted i...