To explain the different secretion kinetics of lysosomal enzymes in Dictyostelium discoideum, previous investigators have hypothesized the existence of a heterogeneous population of lysosomes containing either the enzyme acid phosphatase or other hydrolase enzymes. This proposal predicts that at least two targeting mechanisms exist for lysosomal enzymes in this organism. To begin to investigate...

5. Warren RJ, Moss DW. An automated continuous-monitoring procedure for the determination of acid phosphatase activity in serum. Clin Chim Acta 77, 179-188 (1977). 6. Gundlach G, Muhlhausen B. Untersuchungen zur Kupplung des 1-Naphthols mit Fast-red-TR. J Clin Chem Clin Biochem 18, 603-610 (1980). 7. Babson AL, Read PA. A new assay for prostatic acid phosphatase in serum. Am J Clin Pathol 32, 8...

Amino acid-Fe(II)-chelator complexes exhibit strong antioxidant activity. Taking advantage of the unique spectral characteristics of the complexes formed when Ferrozine (Fz) is used as the chelator, we now show that the primary blue complex (epsilon(max) at 632 nm) decomposes by two independent pathways: (i) a nonoxidative pathway involving dissociation of the amino acid component and formation...

One of the cyr 1 mutants (cyr 1-2) in yeast produced low levels of adenylate cyclase and cyclic AMP at 25 degrees and was unable to derepress acid phosphatase. Addition of cyclic AMP to the cyr1-2 cultures elevated the level of repressible acid phosphatase activity. The bcy1 mutation, which suppresses the cyr1-2 mutation by allowing activity of a cyclic AMP-independent protein kinase, also allo...

All heptoses produce with orcinol in dilute HCl two characteristic colored compounds which differ greatly in their absorption spectra. Three different modifications of this reaction were found useful. Procedure i--To 2 cc. of a solution containing 20 to 100 y per cc. of ketoheptose or 5 times as much of an aldoheptose is added 0.4 cc. of concentrated HCl, sp. gr. 1.19, in a test-tube with a gro...