background and objectives : dna molecular weight marker is widely used in molecular biology experiments incurring considerable costs on low-budget settings. materials and methods : here a pcr-supported procedure is described that uses 10 primer pairs targeting chromosomal dna from the harmless vaccinal bacillus anthracis sterne 34f2 strain as template. a single pcr protocol is used to reproduce...

conclusions the established method has an acceptable performance in quantifying yb-1. in addition, serum yb-1 may aid in the diagnosis of hcc. results the developed method was linear to 150 μg/l of yb-1 with a minimum detection limit of 0.01 μg/l. the average recoveries were between 93.9% and 109.0%. the mean intra- and inter-assay coefficients of variation (cvs) were 4.0-4.8% and 8.2-10.2%, re...

results in a group of adult patients with chb, anxa2 values were far higher than those of the control group (p = 0.001). when we assessed anxa2 levels based on fibrosis stages, serum anxa2 levels of patients with early stage fibrosis (stages 1 - 3) were significantly higher than those of patients with advanced stage fibrosis (stages 4 - 5; p = 0.001). patients and methods this study included 17...

background: the differentiation of infected diabetic foot ulcers (idfu) from non infected diabetic foot ulcers (nidfu) is a challenging issue for clinicians. objectives: recently, procalcitonin (pct) was introduced as a remarkable inflammatory marker. we aimed to evaluate the accuracy of pct in comparison to other inflammatory markers for distinguishing idfu from nidfu. conclusions: these resul...

the effectiveness of irap, remap, ssr, and issr markers were investigated to assess genetic diversity among and within eight medicago sativa l. populations. a total of 101, 119, 117 loci and 31 alleles were amplified using 10 irap, 14 remap, 16 issr and eight ssr primers, respectively. irap markers generated the maximum proportion of polymorphic loci per primer (pplp) while the maximum value of...

in sturgeon aquaculture, where the main purpose is caviar production, a reliable method is needed to separate fish according to gender. currently, due to the lack of external sexual dimorphism, the fish are sexed by an invasive surgical examination of the gonads. development of a non-invasive procedure for sexing fish based on genetic markers is of special interest. in the present study we empl...

association analysis is a useful method for evaluation of significant association between molecular marker and phenotype of trait. this study was performed to evaluate association between traits related with powdery mildew resistance and molecular markers. this investigation was performed using 77 barley genotypes and aflp markers. in phenotypic evaluation, reaction of seedlings to powdery mild...

effects of induced mutation via gamma irradiated with 250 gy dose (in nuclear agriculture research school- nuclear science and technology research institute) on trichoderma harzianum conidiaon chitinase activity by sts molecular marker have been evaluated in this study. among 20 irradiated mutants which selected via improved antagonistic capability against rhizoctonia solani, pcr amplified chit...

in sturgeon aquaculture, where the main purpose is caviar production, a reliable method is needed to separate fish according to gender. currently, due to the lack of external sexual dimorphism, the fish are sexed by an invasive surgical examination of the gonads. development of a non-invasive procedure for sexing fish based on genetic markers is of special interest. in the present study we empl...

a new system is presented for the generation ofrecombinant bacillus subtilis strains without antibioticmarkers. this system is based on two plasmids constructed in escherichia coli. the first plasmid phm30contains an incomplete hisi gene, the last gene in thehistidine biosynthesis operon of b. subtilis and part ofthe genes yvca and yvcb of unkown function flankinghisi at the 3´-end. the spectin...