The envelope glycoprotein G of rabies virus induces neutralizing antibodies, which are important in protection against rabies. This protein was solubilized from purified virus and isolated by differential and sucrose density gradient centrifugation followed by high-performance liquid chromatography. Conditions for solubilization and purification of G were optimized by using immunoblotting and e...

During 2002, 49 states and Puerto Rico reported 7,967 cases of rabies in nonhuman animals and 3 cases in human beings to the Centers for Disease Control and Prevention, an increase of 7.2% from the 7,436 cases in non-human animals and 1 case in a human being reported in 2001. More than 92% (7,375 cases) were in wild animals, whereas 7.4% (592) were in domestic species (compared with 93.3% in wi...

Disease transmission within and among wild and domestic carnivores can have significant impacts on populations, particularly for threatened and endangered species. We used serology to evaluate potential exposure to rabies virus, canine distemper virus (CDV), and canine parvovirus (CPV) for populations of African lions (Panthera leo), African wild dogs (Lycaon pictus), and spotted hyenas (Crocut...

OBJECTIVE To determine the association between cancer chemotherapy and serum canine distemper virus (CDV), canine parvovirus (CPV), and rabies virus antibody titers in tumor-bearing dogs. DESIGN Prospective study. ANIMALS 21 client-owned dogs with various malignancies and 16 client-owned dogs with lymphoma. PROCEDURE In study A, serum antibody titers were measured by use of hemagglutinati...

Genetic and phylogenetic analyses of the region containing the glycoprotein (G) gene, which is related to pathogenicity and antigenicity, and the G-L intergenic region were carried out in 14 Brazilian rabies virus isolates. The isolates were classified as dog-related rabies virus (DRRV) or vampire bat-related rabies virus (VRRV), by nucleoprotein (N) analysis. The nucleotide and amino acid (AA)...

Before the introduction of control programs in the 20th century, rabies in domestic dogs occurred throughout the Western Hemisphere. However, historical records and phylogenetic analysis of multiple virus isolates indicate that, before the arrival of the first European colonizers, rabies virus was likely present only in bats and skunks. Canine rabies was either rare or absent among domestic dog...

The government's program to achieve rabies-free status by 2030 is an initiative accelerate the eradication of rabies cases in Indonesia. Rabies a highly contagious disease with 100% fatality rate, and dogs are main carriers virus. efforts minimize include vaccination susceptible animals, eliminating rabid dogs, implementing dog population management (MPA). Field observations indicate that pract...

The principle underlying the use of labeled antibody in the study of cellular antigens, both natural and foreign, is well understood. Recent reviews have been published by Liu (9), Poetschke (14), Hers (7), and Mims (13). Moreover, a review of the whole field of the use and abuse of fluorescent antibody was published by Beutner in 1961 (1). It is the purpose of this presentation to call attenti...

Five cases of Mokola virus, a lyssavirus related to rabies, are described. The cases occurred in cats from the East London, Pinetown and Pietermaritzburg areas of South Africa from February 1996 to February 1998. Each of the cats was suspected of being rabid and their brains were submitted for laboratory confirmation. Four of the cases were positive, but with atypical fluorescence, and 1 was ne...

BACKGROUND Oral rabies vaccination (ORV) in rabies infected regions should target the primary rabies vector species, which in Lithuania includes raccoon dogs as well as red foxes. Specific investigations on ORV in raccoon dogs are needed e.g. evaluation of vaccine effectiveness under field conditions. The objective of the current study was to investigate the efficacy of the ORV programme 2006-2...