in order to investigate the effect of different temperature of anesthetic (clove tree) and recover (anesthetic-free water) solutions on recovery process, the anesthetic induction times and recovery times of 540 fingerlings of rainbow trout (oncorhynchus mykiss) weighing 3.2 ± 0.2 g in anesthesia/ recovery solutions with cross-combined of temperature of 5,11 and 17 °c as experimental treatments ...

Mammalian studies report that methionine restriction (MR) as a dietary regimen extends life span, delays the onset of age-related diseases and enhances fat oxidation in obese subjects with metabolic syndromes. However, the underlying cellular signalling pathways are poorly understood. Rainbow trout (Oncorhynchus mykiss) is a glucose-intolerant species, providing an excellent model for the study...

The importance of rainbow trout (Onchorhynchus mykiss) in Iran aquaculture industry on one hand, and increasing the mortality of this fish due to outbreaks of infectious diseases, on the other hand, indicate the requirement for more profound understanding the rainbow trout immune system and access to laboratory tools for definitive diagnosis of its diseases. One of the most important defense me...

An important advance in aquatic ecotoxicology is the development and application of biomimetic sampling technology for hydrophobic contaminants. In this paper, accumulation kinetics of five organochlorinated pesticides, i.e. hexachlorobenzene, lindan, aldrin, heptachlor epoxide and 4,4'-DDT, by triolein-containing semipermeable membrane device (triolein-SPMD) and by fish (rainbow trout) were co...

Vitellogenin (VTG) induction has proved to be a valuable biomarker for assessing exposure to environmental estrogens in fish. The widespread use of VTG in this regard has lead to the need for standardized assays to quantify VTG, and monoclonal antibodies have the potential to help accomplish this. A VTG enzyme-linked immunosorbent assay (ELISA) was developed using a monoclonal antibody prepared...

Aflatoxin B1, the most potent hepatic chemical carcinogen known, is activated to the putative product aflatoxin B1-2,3-epoxide via a cytochrome P-450-dependent reaction. Mt. Shasta rainbow trout is the most sensitive species known to the hepatocarcinogenic effects of aflatoxin B1. We have previously isolated and purified a minor form of cytochrome P-450 from this strain of rainbow trout, with a...

Fibroblasts have shown to be an immune competent cell type in mammals. However, little is known about the immunological functions of this cell-type in lower vertebrates. A rainbow trout hypodermal fibroblast cell-line (RTHDF) was shown to be responsive to PAMPs and DAMPs after stimulation with LPS from E. coli, supernatant and debris from sonicated RTHDF cells. LPS was overall the strongest ind...

A 10X rainbow trout bacterial artificial chromosome (BAC) library was constructed to aid in the physical and genetic mapping efforts of the rainbow trout genome. The library was derived from the Swanson clonal line (YY male) and consists of 184,704 clones with an average insert size of 137,500 bp (PFGE) or 118,700 bp (DNA fingerprinting). The clones were gridded onto 10 large nylon membranes to...

The aim of our study was to investigate acute and chronic effects of the oil cleaner Simple Green (SG), crude oil (oil), crude oil and SG mixture (oil-SG) on rainbow trout (Oncorhynchus mykiss) in early development stages (embryos, larvae). The acute effects of SG, oil, and oil-SG mixture on rainbow trout embryos and larvae were investigated. The 96-hour LC50 values for embryos/larvae were: SG ...

Environmental Flow Requirement (EFR) is defined as the flow that is necessary to ensure the existence of habitats in water resources systems. EFR is defined in rivers as flow index commonly. Tennant method is the most popular hydrological method in rivers and based on the historic flow data. The most common method of hydraulic rating method is the wetted perimeter method. Habitat simulation tec...