Purpose TO examine the ability of retinal pigment epithelial (RPE) cells to superantigen to T lymphoc e&t2 resent bacterial es. Methods RPE cells, er interferon gamma (INFg) treated or untreated, were coincubated with Jurkat T-cells, staphylococcal enterotoxin E (SEE), and PMA for 24 hours. Supernatants were collected for interleukin-2 (IL-2) assay. Raji B-calls were assayed for comparison. Bin...

Raji cells were used for the isolation of complement-fixing antigen-antibody complexes from serum. Immune complexes bound to these cells were radiolabeled at the cell surface with lactoperoxidase. The complexes were then eluted from the cells with isotonic citrate buffer pH 3.2 or recovered by immunoprecipitation of cell lysates. The antigen and antibody moieties of the complexes were isolated ...

Purpose TO examine the ability of retinal pigment epithelial (RPE) cells to superantigen to T lymphoc e&t2 resent bacterial es. Methods RPE cells, er interferon gamma (INFg) treated or untreated, were coincubated with Jurkat T-cells, staphylococcal enterotoxin E (SEE), and PMA for 24 hours. Supernatants were collected for interleukin-2 (IL-2) assay. Raji B-calls were assayed for comparison. Bin...

Purpose TO examine the ability of retinal pigment epithelial (RPE) cells to superantigen to T lymphoc e&t2 resent bacterial es. Methods RPE cells, er interferon gamma (INFg) treated or untreated, were coincubated with Jurkat T-cells, staphylococcal enterotoxin E (SEE), and PMA for 24 hours. Supernatants were collected for interleukin-2 (IL-2) assay. Raji B-calls were assayed for comparison. Bin...

We showed previously that human malignant non-Hodgkin’s lymphomas (NHL) degrade extracellular matrix (ECM) components through the action of metalloproteinases and that elevated expression of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) correlated with a poor clinical outcome in patients with NHL. In the present study we sought to investigate whether th...

The B2 antigen characterized by means of a monoclonal antibody (14) is a 140,000 Mr protein expressed only in certain stages of the differentiation of lymphocytes of the B lineage. Here we examine the relationship between B2 and the membrane complement receptor type 2 (CR2) for the complement fragment C3d (11, 12), which is also associated only with B cells. Both phenotypic markers are distribu...

We showed previously that human malignant non-Hodgkin's lymphomas (NHL) degrade extracellular matrix (ECM) components through the action of metalloproteinases and that elevated expression of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) correlated with a poor clinical outcome in patients with NHL. In the present study we sought to investigate whether th...

The alkylating agents represent one of the most important classes of antitumor agents and play a major role in combination with other agents in the curative chemotherapy of selected human cancers. By repeatedly exposing cells to escalating doses of an alkylating agent, we have developed four human tumor cell lines which are relatively stably resistant to the drug with which the culture was trea...

  Introduction: Radioimmunotherapy (RIT) is a very promising new therapy for the treatment of recurrent B-Cell non-Hodgkin's lymphoma (NHL). Iodine-131 is the most frequently used nuclide in clinical RIT, but its usefulness has been limited by dehalogenation of monoclonal antibodies labeled via conventional methods. To circumvent this problem, we have synthesized a tr...

Eighteen patients with Stage 4 B-cell malignancies, which were primarily of intermediate or high grade and progressive despite multiple drug chemotherapy and external irradiation, were treated with fractionated doses of 131I-labeled Lym-1. Lym-1 is an IgG2a monoclonal antibody that was produced by immunizing mice with Raji cell nuclei that originated from a patient with African Burkitt's lympho...