We developed random amplified polymorphic DNA (RAPD) analysis for the assessment of the genetic relationship between cultured populations of the Pacific oyster Crassostrea gigas Thunberg in Hiroshima and Goseong, the largest oyster farming areas in Japan and Korea, respectively. Of 25 arbitrary primers comprising decamer nucleotides of random sequences, polymerase chain reaction amplifications ...

The genus Melipona (Hymenoptera: Apidae; Meliponinae), represented by more than 40 known species, is limited to the neotropical regions of South and Central America, including the Caribean Islands (Camargo, 1979; Michener, 1979, 1990). In Brazil, Melipona quadrifasciata Lep., known as mandaçaia, may be found along the coast side, from the State of Paraíba to the State of Rio Grande do Sul (Mour...

Almond is considered the most common and essential nut grown in rainfed area. It has many species wildly distributed Iraqi Kurdistan region, which are called Chaqala by local people. To understand improve characteristics of cultivated almonds, a comprehensive study wild almonds terms population structure genetic diversity needed to transfer new traits into domesticated almonds. So, twelve diffe...

Introduction The recent development of the random amplified polymorphic DNA (RAPD) marker system (Williams et al., 1990) has spawned considerable interest within the plant breeding community. The simplicity of the assay itself, as compared to restriction fragment length polymorphism (RFLP) analysis, potentially affords the opportunity to apply this marker system to the benefit of both basic and...

Curcuma wenyujin is an important multifunctional medicinal herb in China. Currently, populations of C. wenyujin are decreasing, and wild individuals have almost disappeared from their natural habitats. Moreover, little is known regarding the molecular characteristics of this plant. In this study, we investigated the genetic diversity and variation of five populations of C. wenyujin, using ran-d...

A simple and routine method for the analysis of tissue culture-derived strawberry plants for somaclonal variations is a prerquist for pricise monitring of quality control during rapid mass micropropgation. Similarly, molecular identification of different varieties is an important element for efficient and effective management of strawberry genetic resources. This study reports on the use of RAP...

Five bacterial strains were isolated from the hemocoel of the greater wax moth larvae (Galleria mellonella) infected with the entomopathogenic nematodes: Heterorhabditis bacteriophora HP88, Heterorhabditis indicus RM1 and Heterorhabditis sp (S1), Steinernema abbasi and Steinernema sp. (S II). Strains were identified as Photorhabdus luminescens HRM1, P. luminescens HS1, P. luminescens HP88, Xeno...

Random amplified polymorphic DNA (RAPD) markers were successfully used in discrimination of sexes in Nile tilapia fish (Oreochromis niloticus) using linear discriminant function analysis. The results provide support for the view that major genetical sex determining factors exist in tilapia.

Received fo Jersey Agr Hatch Act, thank P. S Demoranvi this paper regulations indicate thi 1Postdocto 2Associate 3Associate Abstract. Highly variable productivity among Vaccinium macrocarpon (Ait.) Pursh ‘McFarlin’ bogs in Washington has been noted by growers. The fruiting habits of 12 Washington ‘McFarlin’ bogs, ranging from 5.7–28.4 t/ha productivity were characterized. Uprights from each bog...

This study investigated the genetic diversity of morchella species using randomly amplified polymorphic DNA markers. Morchella species are an important group of edible mushrooms belonging to the family Helveliaceae with medicinal and economical significance. In this study we have developed an efficient method of genomic DNA isolation which was amplified by eight RAPD primers to test the polymor...