influenza a virus is now considered to be widespread in poultry and has demonstrated the ability to infect humans in iran. for laboratory diagnosis of these respiratory viruses, it is essential to have rapid methods, able to detect viruses in early stages of the infection in clinical specimens. the real-time reverse-transcription polymerase chain reaction n (rrt-pcr) assay has been established ...

background and objectives: for further confirmation of the previous in-house real-time pcr and cyp 141 target as a rapid and cheap diagnostic technique and a new target for direct detection of mycobacterium tuberculosis , we evaluated and compared the results of smear, culture and real-time pcr in sputa that were collected from 2 health centers. moreover we tried to evaluate the diagnostic accu...

background: cutaneous leishmaniasis (cl) is endemic in southeastern anatolia, mainly in sanliurfa and hatay provinces, and the causative agents are mostly leishmania tropica and less frequently l. infantum . here, we report the first mal-di-tof analyses of leishmania promastigotes obtained from the cultures of two cl cases from osmaniye and hatay provinces who were initially diagnosed by micros...

objective: in this study quantitative expression of mdr1 and hoct1 genes in cml patients and normal people were measured using real-time pcr. materials and methods: to study quantitative expression of these genes by real-time pcr, master-mix with syber green was used. peripheral blood samples from 30 cml patients and 27 normal persons were harvested. real-time pcr results were analyzed with re...

plasma membrane h+-atpase is a major integral membrane protein with a role in various physiological processes including abiotic stress response. to study the effect of nacl on the expression pattern of a gene encoding the plasma membrane h+-atpase, an experiment was carried out in a completely random design with three replications. a pair of specific primers was designed based on the sequence o...

septoria tritici blotch (stb), caused by the ascomycete fungus mycosphaerella graminicola (asexual stage: septoria tritici), is one of the most important foliar diseases of wheat. in this research, quantitative expression analysis of five candidate genes for the induction of resistance to stb (pr-1, bsi, msr, per, and ppi) was conducted in the wheat cultivars ‘seri 82’ (susceptible) and ‘fronta...