To detect the biological agents, the DNA detection is most usable due to the sensitivity and the selectivity. Although the key technology of DNA detection is real-time polymerase chain reaction (PCR), which uses exponential amplification of PCR and real-time detection of the amplified DNA fragments, the long thermal cycling time has been the problem to apply the rapid detection of pathogenic or...

stevens-johnson syndrome (sjs) and toxic epidermal necrolysis (ten) are severe adverse cutaneous reactions to drugs. we describe the case of a 19 year old patient with sjs/ten overlap syndrome, who developed severe interstitial pneumonia after she had received antiepileptic drugs. a cytomegalovirus infection was diagnosed by real time polymerase chain reaction (rt-pcr) detection on bronchoalveo...

Background: Transient and stable transformation of host plants are the common techniques to produce transgenic plants. However, the main drawback of stable transformation is the fact that it takes quite a long time to produce a transgenic line. While, transient gene expression is a quick method to produce recombinant proteins in plants.  Objective: The main goal of the present study was to eva...

Attendance at this year's European Calcified Tissue Society's (ECTS) Congress reveals that the methods used to obtain qPCR results continue to be significantly flawed and that and their reporting remain inadequate.

Real time PCR (quantitative PCR, qPCR) is now a well-established method for the detection, quantification, and typing of different microbial agents in the areas of clinical and veterinary diagnostics and food safety. Although the concept of PCR is relatively simple, there are specific issues in qPCR that developers and users of this technology must bear in mind. These include the use of correct...