نتایج جستجو برای: recombinant protein production
تعداد نتایج: 1809349 فیلتر نتایج به سال:
The homeobox genes are known to play a crucial role in controlling the development of multicellular organisms. The majority of these genes have been determined to express regulatory proteins act as a regulatory protein. These trans-acting factors regulate the expression of proteins that are necessary during the developmental processes throughout the body. TGIFLX/Y is a homeobox gene and it cont...
Background & Aims: Salmonella enterica is a zoonotic pathogen causing typhoid fever in humans and animals. It is an important cause of food borne infections in humans throughout the world. A recent study estimated approximately 22 million cases of typhoid each year with at least 200,000 deaths. FliC encoding flagellin plays a role in pathogenesis and is an important antigen in vaccination....
Aeromonas hydrophila is a gram-negative bacterium which associated with gastrointestinal diseases and septicaemia. This pathogenic bacterium has several virulence factors ranging from pili to the excreted protein which called (Aerolysin) with minor and major effects, respectively. Additionally, Aeromonas hydrophila is a widely distributed bacterium that commonly causes ulcers in cyprinid fish s...
Abstract Background and Objective: One of the proteins shared in all strains of Brucella is 31 kDa surface protein (BCPS31) that could be an appropriate target for immunization and serological diagnosis. Material and Methods: In the present study, BCSP31 produced as a recombinant protein in pET28a (+) expression system was utilized, using ELISA, to detect trace specific antibody (IgG) in brucel...
Address: 1Institute for Biotechnology and Biomedicine, Autonomous University of Barcelona, Bellaterra, 08193 Barcelona, Spain, 2Department of Genetics and Microbiology, Autonomous University of Barcelona, Bellaterra, 08193 Barcelona, Spain, 3CIBER-BBN Networking Centre on Bioengineering, Biomaterials and Nanomedicine, Bellaterra, 08193 Barcelona, Spain, 4University of Natural Resources and Appl...
Aim and Background: By Attention to gene sequence and each amino acid codon in host which express protein, also in order to probable vaccine production against influenza A, a major cause of mortality in a year,this research was carried out. M2 protein is conserved and it can be a proper candidate vaccine for influenza infection. Materials and Methods: Present research was an experimental study....
Abstract Background: The cysteine rich region II of Plasmodium vivax Duffy Binding Protein (PvDBP-II) is essential during merozoite invasion into the human erythrocyte and because of this biological importance, PvDBP-II is a vaccine candidate and a target for protective immunity against vivax malaria. In the present study we improved the yield of this protein by using E. coli (M15/BL21) expr...
Designing appropriate expression vectors is one of the critical steps in the generation of stable cell lines for recombinant protein production. Conventional expression vectors are severely affected by the chromatin environment surrounding their integration site into the host genome, resulting in low expression levels and transgene silencing. In the past, a new generation of expression vectors ...
Increasing interest in recombinant protein technologies for human and animal health applications has spotlighted microalgae as a platform with the potential to meet a large impending demand. Here we describe an algae protein expression system and compare the advantages and disadvantages to other platforms currently operating on a commercial level. High-value recombinant proteins that have been ...
erated by introducing foreign genes into the nuclear genome. Here we focus on plastid transformation as an alternative. Plastids have their own rudimentary genome, the plastome, which encodes around 120 genes [1]. Plastids in green tissues are “chloroplasts”, whose primary function is photosynthesis. In contrast to the nuclear genome with typically two copies of each gene per cell, the plastome...
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