MdMYB1 is a crucial regulator of light-induced anthocyanin biosynthesis and fruit coloration in apple (Malus domestica). In this study, it was found that MdMYB1 protein accumulated in the light but degraded via a ubiquitin-dependent pathway in the dark. Subsequently, the MdCOP1-1 and MdCOP1-2 genes were isolated from apple fruit peel and were functionally characterized in the Arabidopsis (Arabi...

In 1954 Alan Turing, mathematician, war hero, killed himself by biting an apple dipped in poison. Just a few years before, he had thrown to the world an apple of discord, conceiving the possibility of an “electronic child”, a programmable machine that could learn, think, be intelligent and even have a conscience. “Can machines think?” – he had written. He thought that yes, machines would think ...

Pectinase is used commercially in the clarification and extraction of fruit juice from different fruits. Green apples and Red apples obtained from the fruits section of a supermarket, Idiroko road, Ota were surface sterilized and inoculated with Aspergillus niger. The stock culture was subcultured on Sabouraud Dextrose agar plates and 72-hr-old culture of Aspergillus niger served as the inoculu...

Fluorescent proteins (FPs) are widely used in real-time single virus particle studies to visualize, track and quantify the spatial and temporal parameters of viral pathways. However, potential functional differences between the wild type and the FP-tagged virus may specifically affect particular stages in the virus life-cycle. In this work, we genetically modified the E2 spike protein of Sindbi...

MdMYB1 is a crucial regulator of light-induced anthocyanin biosynthesis and fruit coloration in apple (Malus domestica). In this study, it was found that MdMYB1 protein accumulated in the light but degraded via a ubiquitin-dependent pathway in the dark. Subsequently, the MdCOP1-1 and MdCOP1-2 genes were isolated from apple fruit peel and were functionally characterized in the Arabidopsis (Arabi...

Environmental Microbial and Food Safety Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, United States Department of Agriculture, 10300 Baltimore Ave, Beltsville, MD 20705 National Academy of Agricultural Science, Rural Development Administration, Suwon 441-100, Republic of Korea Department of Biosystems Machinery Engineering, Chungnam National University, Dae...

Variations of phenolics, antioxidant activity, and mineral contents in peel and pulp of five apple (Malus domestica Borkh.) cultivars from Pakistan, namely Red Delicious, Golden Delicious, Kashmiri Amri, Kala Kulu and Sky Spur were appraised. The mean extract yield of antioxidant components obtained with 80:20 methanol-water (v/v), was found to be 22.1 g/100 g for peel and 14.2 g/100 g for pulp...

Fruit softening is a complex, genetically programmed and environmentally regulated process, which undergoes biochemical physiological changes during fruit development. The molecular mechanisms that determine these in Chinese cherry [ Cerasus peseudocerasus (Lindl.) G.Don] fruits are still unknown. In the present study, of hard-fleshed ‘Hongfei’ soft-fleshed ‘Pengzhoubai’ varieties were selected...