Protoplasts from four different species of Streptomyces regenerated cells efficiently in hypertonic soft agar medium overlaid on partially dehydrated regeneration medium. The efficiencies of regeneration were strongly dependent upon the incubation temperatures for cell growth and for protoplast regeneration. Cell growth temperatures (before protoplast formation) required for efficient protoplas...

We review what is known about amphibian limb regeneration from the prospective of developing strategies for the induction of regeneration in adult mammals. Prominent in urodele amphibian limb regeneration is the formation of a blastema of undifferentiated cells that goes on to reform the limb. The blastema shares many properties with the developing limb bud; thus, the outgrowth phase of regener...

With the glutathione system that leads to rapid regeneration of reduced lysozyme (Saxena, V. P., and Wetlaufer, D. B. (1971) Biochemistry 9, 5015), reduced pancreatic ribonuclease (RNase) regenerated activity in high yield (greater than 90%) but at a considerably lower rate (t1/2 approximately 75 min). Systematic examination of the effects upon regeneration of the concentrations and ratios of r...

Regeneration requires both potential and instructions for tissue replacement. In planarians, pluripotent stem cells have the potential to produce all new tissue. The identities of the cells that provide regeneration instructions are unknown. Here, we report that position control genes (PCGs) that control regeneration and tissue turnover are expressed in a subepidermal layer of nonneoblast cells...

Regeneration of boneless amputated forearms of adult newts was found to progress at a rate and to a degree comparable to amputated control limbs in which stump bones were not removed. In contrast, regeneration of boneless amputated Xenopus froglet forearms was significantly delayed and did not occur until two to three weeks following amputation. This is in comparison with the initiation of dist...

BACKGROUND Several organs such as the skin and liver have a great capacity for regeneration. However, many approaches only delay the progression of end-stage kidney disease and do not achieve efficient long-term stabilization, let alone regeneration. SUMMARY In mammals, the kidney has an innate but limited capacity for regeneration which can only modify the nephron structure and function but ...

Experimental diabetes caused by pancreatectomy resulted in a marked interference in limb and tail regeneration in adult newts. Under pancreatic insufficiency, throughout the entire period of regeneration, limb and tail regenerates (26 days old) consistently showed sparse population of blastema cells, while older regenerates (58-79 days) exhibited severe abnormalities. Newts with these abnormal ...

Pluridisciplinary approaches led to the notion that fin regeneration is an intricate phenomenon involving epithelial-mesenchymal and reciprocal exchanges throughout the process as well as interactions between ray and interray tissue. The establishment of a blastema after fin amputation is the first event leading to the reconstruction of the missing part of the fin. Here, we review our knowledge...