نتایج جستجو برای: rep pcr

تعداد نتایج: 181497  

Journal: :PloS one 2016
Franziska Tomaschek Paul G Higgins Danuta Stefanik Hilmar Wisplinghoff Harald Seifert

To compare the two Acinetobacter baumannii multi-locus sequence typing (MLST) schemes and to assess their suitability to aid in outbreak analysis we investigated the molecular epidemiology of 99 Acinetobacter baumannii isolates representing outbreak-related and sporadic isolates from 24 hospitals in four different countries (Germany, Poland, Sweden, and Turkey). Pulsed-field gel electrophoresis...

ژورنال: بیماریهای گیاهی 2012
سید محسن تقوی, محمد جواهری, محمود شهابی محمدآبادی

در سال‌های 1387 و 1388 از باغ‌های مو، گلخانه‌های پرورش گل رز و هم‌چنین مزارع چغندرقند در استان‌های فارس و کهگیلویه و بویراحمد بازدید و از گیاهان دارای علایم گال طوقه و ریشه و خاک اطراف آنها نمونه‌برداری و به آزمایشگاه منتقل شد. جداسازی عامل بیماری روی محیط‌های NA، DIM agar، 1A و RS انجام گرفت. پس از خالص‌سازی جدایه‌ها، با انجام آزمون‌های استاندارد باکتری‌شناس...

Journal: :Cellular & molecular biology letters 2003
Katarzyna Baldy-Chudzik Joanna Niedbach Michał Stosik

The rep-PCR fingerprinting method, with the support of ERIC and REP primers, was used to analyse the genomic diversity of 93 E. coli strains isolated from lake water samples drawn at two different depths. The applied UPGMA for DNA analysis did not reveale any genomic similarities between the 48 E. coli strains derived from the subsurface-zone water and the 43 of the bottom-zone water. The consi...

Journal: :Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc 2009
Anina B J Stahel Richard K Hoop Peter Kuhnert Bozena M Korczak

Several bacteria belonging to the family Pasteurellaceae are potential pathogens in rabbits. In particular, Pasteurella multocida is considered to be important, and outbreaks caused by this species result in considerable economic losses in rabbitries. However, Pasteurellaceae spp. isolated from rabbits are poorly characterized, and thus, proper identification of P. multocida isolates from these...

Journal: :International microbiology : the official journal of the Spanish Society for Microbiology 2013
Hamdi H Zahran Rajaa Chahboune Silvia Moreno Eulogio J Bedmar Medhat Abdel-Fattah Manal M Yasser Ahmed M Mahmoud

Fifty four bacterial strains were isolated from root nodules of the grain legumes Cicer arietinum, Lens esculentus, Phaseolus vulgaris, Pisum sativum, and Vicia faba grown in cultivated lands of Beni-Suef Governorate (Egypt). Repetitive extragenic palindromic (REP)-polymerase chain reaction (PCR) clustered the strains into 15 REP-PCR groups. The nearly complete sequence of the 16S rRNA gene fro...

2015
Palmira De Bellis Mariana Tristezza Miriam Haidukowski Francesca Fanelli Angelo Sisto Giuseppina Mulè Francesco Grieco Annie Pfohl-Leszkowicz

Ochratoxin A (OTA) is a mycotoxin with a main nephrotoxic activity contaminating several foodstuffs. In the present report, five soil samples collected from OTA-contaminated vineyards were screened to isolate microorganisms able to biodegrade OTA. When cultivated in OTA-supplemented medium, OTA was converted in OTα by 225 bacterial isolates. To reveal clonal relationships between isolates, mole...

2012
Neelam Taneja Garima Sangar Goutam Chowdhury T. Ramamurthy Arti Mishra Meenakshi Singh Meera Sharma

BACKGROUND & OBJECTIVES Several outbreaks of cholera have been reported in Chandigarh region during a span of seven years from 2002-2008. The genetic characteristics of Vibrio cholerae isolates obtained during these outbreaks have not been adequately studied. The aim of this study was to do molecular typing of V. cholerae isolated from the sporadic and outbreak cases by pulsed-field gel electro...

ژورنال: پژوهش در پزشکی 2013
دبیری, حسین, دورقی, معصومه, قلاوند, زهره, گودرزی, حسین,

Abstract Background: Efflux systems are components of the bacterial membrane that play a role in homeostasis of the cell and extrusion of toxic compounds. Efflux pumps of the resistance nodulation cell division (RND) superfamily, confer multidrug resistance to their host when over-expressed. One of the RND efflux systems, is AdeABC which have been characterized for A. baumannii. Another pump be...

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