www.sciencemag.org SCIENCE VOL 329 2 JULY 2010 5 COVER Electron micrograph of Mycoplasma mycoides JCVI-syn1.0 cells (magnifi cation ~25,000×). These cells were produced following transplantation of a 1.08–megabase pair synthetic M. mycoides genome into M. capricolum recipient cells. The cells are controlled by the synthetic genome, exhibit the expected phenotypic properties, and are capable of ...

A common theme in medical microbiology is that the amount of amino acid sequence variation in proteins that are targets of the host immune system greatly exceeds that found in metabolic enzymes or other housekeeping proteins. Twenty-four Mycobacterium tuberculosis genes coding for targets of the host immune system were sequenced in 16 strains representing the breadth of genomic diversity in the...

resistance gene analog polymorphism (rgap) markers linked to fusarium head blight resistance (fhb) and co-localize with qfhs.ndsu-3bs were identified using f3 plants and f3:5 lines derived from a ‘wangshuibai’ (resistant) / ‘seri82’ (susceptible) cross. the mapping populations were genotyped using 50 degenerate primers designed based on the known r genes. out of the 50 designed primer combinati...

Six genes, including UL32, have been implicated in the cleavage and packaging of herpesvirus DNA into preassembled capsids. We have isolated a UL32 insertion mutant which is capable of near-wild-type levels of viral DNA synthesis; however, the mutant virus is unable to cleave and package viral DNA, consistent with the phenotype of a previously isolated temperature-sensitive herpes simplex virus...

SRC kinases and PKR are intracellular protein kinases, which play key roles in intracellular viral replication. In this research, the effect of SRC kinase inhibition and PKR activation and inhibition on replication of coxsakievirus (CVB3), an entrovirus of the family picornaviridae – causative agents of fatal myocarditis, was studied. Vero and Hela cells were cultured and infected with CVB3 in ...

As grow as the data-intensive applications in cloud computing day after day, data popularity in this environment becomes critical and important. Hence to improve data availability and efficient accesses to popular data, replication algorithms are now widely used in distributed systems. However, most of them only replicate the static number of replicas on some requested chosen sites and it is ob...

1. Kimura K, Suzuki S, Wachino J et al. First molecular characterization of group B streptococci with reduced penicillin susceptibility. Antimicrob Agents Chemother 2008; 52: 2890–7. 2. Nagano N, Nagano Y, Kimura K et al. Genetic heterogeneity in pbp genes among clinically isolated group B streptococci with reduced penicillin susceptibility. Antimicrob Agents Chemother 2008; 52: 4258–67. 3. Jon...